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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1975-12-29
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pubmed:abstractText |
The enrichment of tRNA at specific sites with carbon-13 has been accomplished in vivo using a mutant of Escherichia coli. A relaxed strain of E. coli auxotrophic for methionine was grown in a specifically defined medium supplemented with either [14C] or [13C]-methyl labeled methionine. Cells were collected at the end of the log-phase of growth and tRNA was extracted. Analysis of the radioactivity of the [14C]-labeled tRNA established an incorporation ratio of three labeled carbons per tRNA molecule. Incorporation of the [14C]-label in vivo was confined to the methylation of nucleotides as determined by thin layer chromatography of nucleotides resulting from a ribonuclease digestion of [14C]-labeled tRNA. The carbon-13 NMR spectrum of [13C]-enriched tRNA indicated a similar degree of incorporation into the methylated nucleotides by the substantial enhancement of [13C]-methyl NMR signals only. Assignment of signals has been made for the methyl groups of ribothymidine and N7-methylguanosine in E. coli tRNA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4369721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4558659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4584321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4584885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4589310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4590172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4594296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4594852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4601809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4606505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4609465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4689778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4831072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4862513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1101225-4902813
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1503-12
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1101225-Carbon Isotopes,
pubmed-meshheading:1101225-Escherichia coli,
pubmed-meshheading:1101225-Guanosine,
pubmed-meshheading:1101225-Magnetic Resonance Spectroscopy,
pubmed-meshheading:1101225-Nucleic Acid Conformation,
pubmed-meshheading:1101225-RNA, Bacterial,
pubmed-meshheading:1101225-RNA, Transfer,
pubmed-meshheading:1101225-Ribose,
pubmed-meshheading:1101225-Thymidine
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pubmed:year |
1975
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pubmed:articleTitle |
Utilization of an Escherichia coli mutant for carbon-13 enrichment of tRNA for NMR studies.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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