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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2000-10-23
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pubmed:abstractText |
Vascular endothelial growth factor (VEGF) increases hydraulic conductivity (L(p)) by stimulating Ca(2+) influx into endothelial cells. To determine whether VEGF-mediated Ca(2+) influx is stimulated by release of Ca(2+) from intracellular stores, we measured the effect of Ca(2+) store depletion on VEGF-mediated increased L(p) and endothelial intracellular Ca(2+) concentration ([Ca(2+)](i)) of frog mesenteric microvessels. Inhibition of Ca(2+) influx by perfusion with NiCl(2) significantly attenuated VEGF-mediated increased [Ca(2+)](i). Depletion of Ca(2+) stores by perfusion of vessels with thapsigargin did not affect the VEGF-mediated increased [Ca(2+)](i) or the increase in L(p). In contrast, ATP-mediated increases in both [Ca(2+)](i) and L(p) were inhibited by thapsigargin perfusion, demonstrating that ATP stimulated store-mediated Ca(2+) influx. VEGF also increased Mn(2+) influx after perfusion with thapsigargin, whereas ATP did not. These data showed that VEGF increased [Ca(2+)](i) and L(p) even when Ca(2+) stores were depleted and under conditions that prevented ATP-mediated increases in [Ca(2+)](i) and L(p). This suggests that VEGF acts through a Ca(2+) store-independent mechanism, whereas ATP acts through Ca(2+) store-mediated Ca(2+) influx.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Manganese,
http://linkedlifedata.com/resource/pubmed/chemical/Nickel,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/nickel chloride
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0363-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
279
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H1625-34
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11009449-Adenosine Triphosphate,
pubmed-meshheading:11009449-Animals,
pubmed-meshheading:11009449-Calcium,
pubmed-meshheading:11009449-Calcium Channel Blockers,
pubmed-meshheading:11009449-Capillary Permeability,
pubmed-meshheading:11009449-Endothelial Growth Factors,
pubmed-meshheading:11009449-Endothelium, Vascular,
pubmed-meshheading:11009449-Enzyme Inhibitors,
pubmed-meshheading:11009449-Intracellular Membranes,
pubmed-meshheading:11009449-Lymphokines,
pubmed-meshheading:11009449-Male,
pubmed-meshheading:11009449-Manganese,
pubmed-meshheading:11009449-Microcirculation,
pubmed-meshheading:11009449-Nickel,
pubmed-meshheading:11009449-Osmolar Concentration,
pubmed-meshheading:11009449-Rana pipiens,
pubmed-meshheading:11009449-Rana temporaria,
pubmed-meshheading:11009449-Splanchnic Circulation,
pubmed-meshheading:11009449-Thapsigargin,
pubmed-meshheading:11009449-Vascular Endothelial Growth Factor A,
pubmed-meshheading:11009449-Vascular Endothelial Growth Factors
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pubmed:year |
2000
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pubmed:articleTitle |
VEGF and ATP act by different mechanisms to increase microvascular permeability and endothelial [Ca(2+)](i).
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pubmed:affiliation |
Department of Physiology, University of Bristol, Bristol BS2 8EJ, LE2 7LX United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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