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rdf:type |
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lifeskim:mentions |
umls-concept:C0020885,
umls-concept:C0037083,
umls-concept:C0205266,
umls-concept:C0999699,
umls-concept:C1140999,
umls-concept:C1267092,
umls-concept:C1412517,
umls-concept:C1423613,
umls-concept:C1710082,
umls-concept:C1720675,
umls-concept:C1879547,
umls-concept:C1880177
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pubmed:issue |
9
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pubmed:dateCreated |
2001-2-2
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pubmed:abstractText |
To elucidate the possible role of Rho A/Rho-kinase on lysophosphatidic acid (LPA)-induced contraction in intact guinea-pig ileal smooth muscle, we examined effects of pretreatment with a specific inhibitor of Rho-kinase (Y-27632) on the LPA-induced contraction and MLC20 phosphorylation. In addition, we investigated whether LPA actually elicits an activation of Rho A by studying subcellular distribution of Rho A in unstimulated and stimulated smooth muscles by LPA. LPA induced a less intense, but sustained, contraction compared with ACh, and was accompanied by significant increases in MLC20 phosphorylation. The effects of LPA on tension and MLC20 phosphorylation were inhibited by Y-27632. The ACh-induced contraction, but not increases in MLC20 phosphorylation, was partially inhibited by Y-27632. High K+-induced contraction was unaffected by the inhibitor. LPA stimulated translocation of Rho A from the cytosol to the membrane fraction of the muscle. Translocation of Rho A was also induced by ACh and high K+. These results suggest that LPA-induced contraction of intact ileal smooth muscle is dominated through activation of Rho A and Rho-kinase and subsequent increases in MLC20 phosphorylation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Amides,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Relaxants, Central,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Y 27632,
http://linkedlifedata.com/resource/pubmed/chemical/rho-Associated Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/rhoA GTP-Binding Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0008-4212
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
729-36
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11007536-Acetylcholine,
pubmed-meshheading:11007536-Amides,
pubmed-meshheading:11007536-Animals,
pubmed-meshheading:11007536-Guinea Pigs,
pubmed-meshheading:11007536-Ileum,
pubmed-meshheading:11007536-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:11007536-Lysophospholipids,
pubmed-meshheading:11007536-Male,
pubmed-meshheading:11007536-Muscle, Smooth,
pubmed-meshheading:11007536-Muscle Contraction,
pubmed-meshheading:11007536-Muscle Relaxants, Central,
pubmed-meshheading:11007536-Phosphorylation,
pubmed-meshheading:11007536-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11007536-Pyridines,
pubmed-meshheading:11007536-Signal Transduction,
pubmed-meshheading:11007536-Vasodilator Agents,
pubmed-meshheading:11007536-rho-Associated Kinases,
pubmed-meshheading:11007536-rhoA GTP-Binding Protein
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pubmed:year |
2000
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pubmed:articleTitle |
Activation of Rho signaling contributes to lysophosphatidic acid-induced contraction of intact ileal smooth muscle of guinea-pig.
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pubmed:affiliation |
Department of Pharmacology, Nagoya City University Medical School, Japan. mkawabe@med.nagoya-cu.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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