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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-9-27
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pubmed:abstractText |
The expression of human purinergic P2 receptors (P2X1-7 and P2Y1-11) as well as the ecto-enzymes apyrase (CD39) and 5'-nucleotidase (CD73) was investigated on the nucleic acid level during granulocytic and monocytic differentiation of HL60 cells and on peripheral human blood leukocytes. RT-PCR and dot-blot hybridization assays indicated that mRNA transcripts of all analyzed P2 receptors apart from the P2X3 receptor were expressed during myeloid development of HL60 cells, showing a distinct regulation during the course of differentiation. In blood leukocytes, transcripts of P2X5, P2X7 and all P2Y receptors, except for P2Y6, receptor were found. CD39 and CD73 showed a marked upregulation during myeloid maturation. Functional analysis of P2 receptor-mediated intracellular Ca(2+)-increase after stimulation with ATP revealed no change during granulocytic differentiation, but showed a strong attenuation in both potency and efficacy during monocytic development of HL60 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5'-Nucleotidase,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Apyrase,
http://linkedlifedata.com/resource/pubmed/chemical/CD39 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Glyceraldehyde-3-Phosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/P2RX7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/P2RY1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/P2RY11 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2X,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2X7,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2Y1
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
1492
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
127-38
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11004484-5'-Nucleotidase,
pubmed-meshheading:11004484-Adenosine Triphosphatases,
pubmed-meshheading:11004484-Adenosine Triphosphate,
pubmed-meshheading:11004484-Antigens, CD,
pubmed-meshheading:11004484-Apyrase,
pubmed-meshheading:11004484-Calcium,
pubmed-meshheading:11004484-Cell Differentiation,
pubmed-meshheading:11004484-Gene Expression,
pubmed-meshheading:11004484-Glyceraldehyde-3-Phosphate Dehydrogenases,
pubmed-meshheading:11004484-HL-60 Cells,
pubmed-meshheading:11004484-Humans,
pubmed-meshheading:11004484-Immunoblotting,
pubmed-meshheading:11004484-RNA, Messenger,
pubmed-meshheading:11004484-Receptors, Purinergic P2,
pubmed-meshheading:11004484-Receptors, Purinergic P2X,
pubmed-meshheading:11004484-Receptors, Purinergic P2X7,
pubmed-meshheading:11004484-Receptors, Purinergic P2Y1
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pubmed:year |
2000
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pubmed:articleTitle |
Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL60 cells.
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pubmed:affiliation |
Department of Biochemistry, University of Erlangen, Fahrstr. 17, 91054 Erlangen, Germany.
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pubmed:publicationType |
Journal Article
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