| pubmed-article:11003979 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C0242692 | lld:lifeskim |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C1704336 | lld:lifeskim |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C0079904 | lld:lifeskim |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C0205615 | lld:lifeskim |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:11003979 | lifeskim:mentions | umls-concept:C1517945 | lld:lifeskim |
| pubmed-article:11003979 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:11003979 | pubmed:dateCreated | 2000-11-7 | lld:pubmed |
| pubmed-article:11003979 | pubmed:abstractText | Nuclear factor-kappaB (NF-kappaB) regulates the transcription of a variety of genes involved in immune responses, cell growth, and cell death. However, the role of NF-kappaB in muscle biology is poorly understood. We recently reported that tumor necrosis factor-alpha (TNF-alpha) rapidly activates NF-kappaB in differentiated skeletal muscle myotubes and that TNF-alpha acts directly on the muscle cell to induce protein degradation. In the present study, we ask whether NF-kappaB mediates the protein loss induced by TNF-alpha. We addressed this problem by creating stable, transdominant negative muscle cell lines. C2C12 myoblasts were transfected with viral plasmid constructs that induce overexpression of mutant I-kappaBalpha proteins that are insensitive to degradation via the ubiquitin-proteasome pathway. These mutant proteins selectively inhibit NF-kappaB activation. We found that differentiated myotubes transfected with the empty viral vector (controls) underwent a drop in total protein content and in fast-type myosin heavy-chain content during 72 h of exposure to TNF-alpha. In contrast, total protein and fast-type myosin heavy-chain levels were unaltered by TNF-alpha in the transdominant negative cell lines. TNF-alpha did not induce apoptosis in any cell line, as assessed by DNA ladder and annexin V assays. These data indicate that NF-kappaB is an essential mediator of TNF-alpha-induced catabolism in differentiated muscle cells. | lld:pubmed |
| pubmed-article:11003979 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11003979 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11003979 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11003979 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11003979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11003979 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11003979 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:11003979 | pubmed:issn | 0363-6119 | lld:pubmed |
| pubmed-article:11003979 | pubmed:author | pubmed-author:LiY PYP | lld:pubmed |
| pubmed-article:11003979 | pubmed:author | pubmed-author:ReisM OMO | lld:pubmed |
| pubmed-article:11003979 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11003979 | pubmed:volume | 279 | lld:pubmed |
| pubmed-article:11003979 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11003979 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11003979 | pubmed:pagination | R1165-70 | lld:pubmed |
| pubmed-article:11003979 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:11003979 | pubmed:meshHeading | pubmed-meshheading:11003979... | lld:pubmed |
| pubmed-article:11003979 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11003979 | pubmed:articleTitle | NF-kappaB mediates the protein loss induced by TNF-alpha in differentiated skeletal muscle myotubes. | lld:pubmed |
| pubmed-article:11003979 | pubmed:affiliation | Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA. | lld:pubmed |
| pubmed-article:11003979 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11003979 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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