Source:http://linkedlifedata.com/resource/pubmed/id/11003600
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2000-10-12
|
| pubmed:databankReference | |
| pubmed:abstractText |
We identified two ClC-2 clones in a guinea pig intestinal epithelial cDNA library, one of which carries a 30-bp deletion in the NH(2) terminus. PCR using primers encompassing the deletion gave two products that furthermore were amplified with specific primers confirming their authenticity. The corresponding genomic DNA sequence gave a structure of three exons and two introns. An internal donor site occurring within one of the exons accounts for the deletion, consistent with alternative splicing. Expression of the variants gpClC-2 and gpClC-2Delta77-86 in HEK-293 cells generated inwardly rectifying chloride currents with similar activation characteristics. Deactivation, however, occurred with faster kinetics in gpClC-2Delta77-86. Site-directed mutagenesis suggests that a protein kinase C-mediated phosphorylation consensus site lost in gpClC-2Delta77-86 is not responsible for the observed change. The deletion-carrying variant is found in most tissues examined, and it appears more abundant in proximal colon, kidney, and testis. The presence of a splice variant of ClC-2 modified in its NH(2)-terminal domain could have functional consequences in tissues where their relative expression levels are different.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0363-6143
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
279
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
C1198-210
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11003600-Alternative Splicing,
pubmed-meshheading:11003600-Animals,
pubmed-meshheading:11003600-Base Sequence,
pubmed-meshheading:11003600-Cell Line,
pubmed-meshheading:11003600-Chloride Channels,
pubmed-meshheading:11003600-Exons,
pubmed-meshheading:11003600-Gene Expression,
pubmed-meshheading:11003600-Guinea Pigs,
pubmed-meshheading:11003600-Humans,
pubmed-meshheading:11003600-Intestinal Mucosa,
pubmed-meshheading:11003600-Introns,
pubmed-meshheading:11003600-Ion Channel Gating,
pubmed-meshheading:11003600-Kidney,
pubmed-meshheading:11003600-Male,
pubmed-meshheading:11003600-Membrane Potentials,
pubmed-meshheading:11003600-Molecular Sequence Data,
pubmed-meshheading:11003600-Organ Specificity,
pubmed-meshheading:11003600-Patch-Clamp Techniques,
pubmed-meshheading:11003600-Protein Structure, Tertiary,
pubmed-meshheading:11003600-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11003600-Sequence Analysis, DNA,
pubmed-meshheading:11003600-Sequence Homology, Amino Acid,
pubmed-meshheading:11003600-Sequence Homology, Nucleic Acid,
pubmed-meshheading:11003600-Transfection
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Splice variants of a ClC-2 chloride channel with differing functional characteristics.
|
| pubmed:affiliation |
Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago-7, Chile. pcid@cecs.cl
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|