rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
4
|
pubmed:dateCreated |
2000-12-20
|
pubmed:abstractText |
Human immunodeficiency virus type 1 (HIV-1) virion is known to carry a number of cellular components including cellular topoisomerase I. Previously, we have demonstrated that topoisomerase I enhances HIV-1 cDNA synthesis in reverse transcription (RT) assays in vitro. In the present study, we have produced six monoclonal antibodies (MAbs) against human topoisomerase I. The MAbs suppressed nicking/closing of supercoiled DNA and cDNA synthesis in an endogenous reverse transcription (ERT) assay using a detergent-disrupted HIV-1 virion. Thus, the results suggest that topoisomerase I plays an important role in RNA-directed DNA polymerization.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0272-457X
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
19
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
331-4
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:11001406-Animals,
pubmed-meshheading:11001406-Antibodies, Monoclonal,
pubmed-meshheading:11001406-Antibody Specificity,
pubmed-meshheading:11001406-Blotting, Southern,
pubmed-meshheading:11001406-Cell Line,
pubmed-meshheading:11001406-DNA, Complementary,
pubmed-meshheading:11001406-DNA, Viral,
pubmed-meshheading:11001406-DNA Replication,
pubmed-meshheading:11001406-DNA Topoisomerases, Type I,
pubmed-meshheading:11001406-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11001406-Female,
pubmed-meshheading:11001406-HIV-1,
pubmed-meshheading:11001406-HeLa Cells,
pubmed-meshheading:11001406-Humans,
pubmed-meshheading:11001406-Mice,
pubmed-meshheading:11001406-Mice, Inbred BALB C,
pubmed-meshheading:11001406-RNA, Viral,
pubmed-meshheading:11001406-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11001406-Transfection,
pubmed-meshheading:11001406-Virus Replication
|
pubmed:year |
2000
|
pubmed:articleTitle |
Monoclonal antibodies against topoisomerase I suppressed DNA relaxation and HIV-1 cDNA synthesis.
|
pubmed:affiliation |
Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. htakahas@nih.go.jp
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|