Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-12-20
pubmed:abstractText
Human immunodeficiency virus type 1 (HIV-1) virion is known to carry a number of cellular components including cellular topoisomerase I. Previously, we have demonstrated that topoisomerase I enhances HIV-1 cDNA synthesis in reverse transcription (RT) assays in vitro. In the present study, we have produced six monoclonal antibodies (MAbs) against human topoisomerase I. The MAbs suppressed nicking/closing of supercoiled DNA and cDNA synthesis in an endogenous reverse transcription (ERT) assay using a detergent-disrupted HIV-1 virion. Thus, the results suggest that topoisomerase I plays an important role in RNA-directed DNA polymerization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0272-457X
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11001406-Animals, pubmed-meshheading:11001406-Antibodies, Monoclonal, pubmed-meshheading:11001406-Antibody Specificity, pubmed-meshheading:11001406-Blotting, Southern, pubmed-meshheading:11001406-Cell Line, pubmed-meshheading:11001406-DNA, Complementary, pubmed-meshheading:11001406-DNA, Viral, pubmed-meshheading:11001406-DNA Replication, pubmed-meshheading:11001406-DNA Topoisomerases, Type I, pubmed-meshheading:11001406-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:11001406-Female, pubmed-meshheading:11001406-HIV-1, pubmed-meshheading:11001406-HeLa Cells, pubmed-meshheading:11001406-Humans, pubmed-meshheading:11001406-Mice, pubmed-meshheading:11001406-Mice, Inbred BALB C, pubmed-meshheading:11001406-RNA, Viral, pubmed-meshheading:11001406-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11001406-Transfection, pubmed-meshheading:11001406-Virus Replication
pubmed:year
2000
pubmed:articleTitle
Monoclonal antibodies against topoisomerase I suppressed DNA relaxation and HIV-1 cDNA synthesis.
pubmed:affiliation
Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. htakahas@nih.go.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't