Linked Life Data

10999850

Source:http://linkedlifedata.com/resource/pubmed/id/10999850

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2000-10-16
pubmed:abstractText
Prostanoids play an important role in the regulation of parturition. All reproductive tissues, including fetal membranes, decidua, and myometrium, have the capacity to synthesize prostanoids, and fetal membranes have been shown to express elevated levels of cyclooxygenase-2 (Cox-2) at the onset of labor. We have now investigated the expression of Cox-2 in human myometrium. Myometrial samples collected from women in labor during lower segment cesarean section expressed 15-fold higher levels of Cox-2 messenger ribonucleic acid (mRNA) compared to myometrial specimens collected from women not in labor, as detected by Northern blot analysis. Immunohistochemical detection of Cox-2 protein showed cytoplasmic staining in the smooth muscle cells of the myometrium. Cultured myometrial cells expressed low levels of Cox-2 mRNA under baseline conditions, but interleukin-1beta (IL-1beta) caused a 17-fold induction of expression of the Cox-2 transcript after incubation for 6 h. IL-1beta also induced expression of biologically active Cox-2 protein, as detected by immunofluorescence, Western blot analysis, and measuring the conversion of arachidonic acid to prostanoids in the presence and absence of a Cox-2-selective inhibitor, NS-398. PGE2 receptor subtype EP2 mRNA was expressed in cultured myometrial smooth muscle cells, whereas transcripts for EP1, EP3, EP4, FP, and IP were low or below the detection limit as measured by Northern blot analysis. However, IL-1beta stimulated expression of EP4 receptor mRNA. Our data suggest that expression of Cox-2 transcript is elevated at the onset of labor in myometrial smooth muscle cells, which may depend on induction by cytokines. As, in addition to Cox-2, the expression of prostanoid receptors is regulated, not only the production of prostanoids, but also responsiveness to them, may be modulated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-972X
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3468-75
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10999850-Adult, pubmed-meshheading:10999850-Blotting, Northern, pubmed-meshheading:10999850-Blotting, Western, pubmed-meshheading:10999850-Cells, Cultured, pubmed-meshheading:10999850-Cyclooxygenase 2, pubmed-meshheading:10999850-Cytokines, pubmed-meshheading:10999850-Epoprostenol, pubmed-meshheading:10999850-Female, pubmed-meshheading:10999850-Fluorescent Antibody Technique, pubmed-meshheading:10999850-Humans, pubmed-meshheading:10999850-Immunohistochemistry, pubmed-meshheading:10999850-Isoenzymes, pubmed-meshheading:10999850-Membrane Proteins, pubmed-meshheading:10999850-Myometrium, pubmed-meshheading:10999850-Pregnancy, pubmed-meshheading:10999850-Prostaglandin-Endoperoxide Synthases, pubmed-meshheading:10999850-Prostaglandins E, pubmed-meshheading:10999850-Prostaglandins F, pubmed-meshheading:10999850-RNA, Messenger, pubmed-meshheading:10999850-Receptors, Prostaglandin
pubmed:year
2000
pubmed:articleTitle
Expression of cyclooxygenase-2 and prostanoid receptors by human myometrium.
pubmed:affiliation
Department of Obstetrics and Gynecology, Helsinki University Central Hospital and University of Helsinki, Finland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't