Source:http://linkedlifedata.com/resource/pubmed/id/10996468
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2000-10-23
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| pubmed:abstractText |
High levels of D-aspartate occur in the brain and endocrine glands, such as pineal, adrenal and pituitary. In the brain, D-aspartate levels are highest in embryonic and early postnatal stages. Notably high levels occur in the early postnatal cortical plate and subventricular zone of the cerebral cortical cultures, implying a role in development. In embryonic neuronal primary culture cells, we detected high levels of endogenous D-aspartate and demonstrated biosynthesis of [14C]D-aspartate using [14C]L-aspartate as precursor. Synthesis of D-aspartate in cell cultures is inhibited by amino-oxyacetic acid, an inhibitor of pyridoxal phosphate-dependent enzymes. In the rat adrenal medulla, D-aspartate is depleted by treatment of the animals with intraperitoneal nicotine injections. In adrenal slices, D-aspartate is released by depolarization with KCl or acetylcholine, implying physiological release by activation of the cholinergic innervation of the adrenal. Our characterization of D-aspartate ontogeny, biosynthesis and depolarization-induced release implies specific physiological roles for this amino acid.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Aminooxyacetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotine,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Chloride
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0306-4522
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
100
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
183-9
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| pubmed:dateRevised |
2009-11-3
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| pubmed:meshHeading |
pubmed-meshheading:10996468-Acetylcholine,
pubmed-meshheading:10996468-Adrenal Glands,
pubmed-meshheading:10996468-Aging,
pubmed-meshheading:10996468-Aminooxyacetic Acid,
pubmed-meshheading:10996468-Animals,
pubmed-meshheading:10996468-Animals, Newborn,
pubmed-meshheading:10996468-Aspartic Acid,
pubmed-meshheading:10996468-Cells, Cultured,
pubmed-meshheading:10996468-Electrophysiology,
pubmed-meshheading:10996468-Embryo, Mammalian,
pubmed-meshheading:10996468-Embryonic and Fetal Development,
pubmed-meshheading:10996468-Endocrine Glands,
pubmed-meshheading:10996468-Enzyme Inhibitors,
pubmed-meshheading:10996468-Injections, Intraperitoneal,
pubmed-meshheading:10996468-Neurons,
pubmed-meshheading:10996468-Nicotine,
pubmed-meshheading:10996468-Potassium Chloride,
pubmed-meshheading:10996468-Rats,
pubmed-meshheading:10996468-Rats, Sprague-Dawley,
pubmed-meshheading:10996468-Tissue Distribution
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| pubmed:year |
2000
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| pubmed:articleTitle |
D-aspartate disposition in neuronal and endocrine tissues: ontogeny, biosynthesis and release.
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| pubmed:affiliation |
Departamento de Bioquimica, ICB/CCS, Universidade Federal do Rio de Janeiro, RJ 21941-590, Rio de Janeiro, Brazil.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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