Linked Life Data

10996021

Source:http://linkedlifedata.com/resource/pubmed/id/10996021

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-11-15
pubmed:abstractText
Interleukin-18 (IL-18) is a novel proinflammatory cytokine. Most of the immune and inflammatory genes induced by IL-18 are nuclear factor-kappaB (NF-kappaB)- and activator protein-1 (AP-1)-regulated. Interleukin-1 receptor-associated kinase-1 (IRAK-1) has recently been shown to be involved in IL-18-stimulated activation of NF-kappaB and AP-1. The purpose of this study is to investigate the effects of preventing IRAK-1 expression by antisense IRAK-1 oligodeoxynucleotide (ODN) on IL-18-stimulated activation of NF-kappaB and AP-1. Semiquantitative reverse transcription-PCR (RT-PCR) and western blot analysis revealed that antisense IRAK-1 ODN inhibited IRAK-1 mRNA and protein expression (P<0.01). As a result, antisense IRAK-1 ODN attenuated IL-18-induced activation of NF-kappaB and AP-1 as measured by sandwich ELISA in a concentration (1-8 microg ml(-1))- and time (5-24 h)-dependent fashion. These data suggest that antisense IRAK-1 ODN may share a role in the design of antiinflammatory therapeutics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0162-3109
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
241-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Antisense IRAK-1 oligonucleotide blocks activation of NF-kappa B and AP-1 induced by IL-18.
pubmed:affiliation
Institute of Pharmaceutic Sciences, The First Military Medical University, 510515, Guang Zhou, People's Republic of China.
pubmed:publicationType
Journal Article