Source:http://linkedlifedata.com/resource/pubmed/id/10989184
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2000-11-7
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pubmed:abstractText |
The effect of porcine reproductive and respiratory syndrome (PRRS) virus infection on the synthesis and secretion of TNF-alpha and other pro-inflammatory cytokines by porcine alveolar macrophages (PAM) was investigated as well as the effect that TNF-alpha has on the replication of this virus. A clear reduction of phorbol myristate acetate (PMA)-induced expression of TNF-alpha mRNA was observed in cells incubated with PRRS virus. Moreover, the presence of PRRS virus also induced a decrease in IL-1 alpha and MIP-1 beta mRNAs expression with respect to PMA-stimulated uninfected cells. According to these results, exposure to the PRRS virus led to a reduction of the TNF-alpha protein in supernatants of PMA-stimulated PAM. On the other hand, addition of recombinant porcine TNF-alpha to cultures clearly reduced virus replication; however the addition of TNF-alpha to cultures containing IFN-alpha did not result in a further reduction of the produced by IFN-alpha alone. This indicates the lack of synergy in the effect of these cytokines on viral replication.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0168-1702
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
41-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10989184-Animals,
pubmed-meshheading:10989184-Base Sequence,
pubmed-meshheading:10989184-DNA Primers,
pubmed-meshheading:10989184-Down-Regulation,
pubmed-meshheading:10989184-Macrophages, Alveolar,
pubmed-meshheading:10989184-Porcine Reproductive and Respiratory Syndrome,
pubmed-meshheading:10989184-Porcine respiratory and reproductive syndrome virus,
pubmed-meshheading:10989184-RNA, Messenger,
pubmed-meshheading:10989184-Recombinant Proteins,
pubmed-meshheading:10989184-Swine,
pubmed-meshheading:10989184-Tumor Necrosis Factor-alpha,
pubmed-meshheading:10989184-Virus Replication
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pubmed:year |
2000
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pubmed:articleTitle |
Porcine reproductive and respiratory syndrome (PRRS) virus down-modulates TNF-alpha production in infected macrophages.
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pubmed:affiliation |
CISA-INIA, Valdeolmos 28130, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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