Linked Life Data

10987503

Source:http://linkedlifedata.com/resource/pubmed/id/10987503

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2001-2-2
pubmed:abstractText
Loxoscelism or necrotic arachnidism are terms used to describe lesions and reactions induced by bites (envenomation) from spiders of the genus Loxosceles. Envenomation has been reported to provoke dermonecrosis and haemorrhage at the bite site and haemolysis, disseminated intravascular coagulation and renal failure. The purpose of this work was to study the effect of the venom of the brown spider Loxosceles intermedia on basement membrane structures and on its major constituent molecules. Light microscopy observations showed that L. intermedia venom obtained through electric shock, which reproduces two major signals of Loxoscelism in the laboratory, exhibits activity toward basement membrane structures in mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Basement degradation was seen by a reduced periodic acid-Schiff (PAS) and alcian blue staining as well as by a reduced immunostaining for laminin when compared to control experiments. Electron microscopy studies confirmed the above results, showing the action of the venom on EHS-basement membranes and demonstrating that these tissue structures are susceptible to the venom. Using purified components of the basement membrane, we determined through SDS-PAGE and agarose gel that the venom is not active toward laminin or type IV collagen, but is capable of cleaving entactin and endothelial heparan sulphate proteoglycan. In addition, when EHS tissue was incubated with venom we detected a release of laminin into the supernatant, corroborating the occurrence of some basement membrane disruption. The venom-degrading effect on entactin was blocked by 1, 10-phenanthroline, but not by other protease inhibitors such as PMSF, NEM or pepstatin-A. By using light microscopy associated with PAS staining we were able to identify that 1,10-phenanthroline also inhibits EHS-basement membrane disruption evoked by venom, corroborating that a metalloprotease of venom is involved in these effects. Degradation of these extracellular matrix molecules and the observed susceptibility of the basement membrane could lead to loss of vessel and glomerular integrity, resulting in haemorrhage and renal problems after envenomation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0018-2214
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
397-408
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10987503-Animals, pubmed-meshheading:10987503-Basement Membrane, pubmed-meshheading:10987503-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:10987503-Heparitin Sulfate, pubmed-meshheading:10987503-Humans, pubmed-meshheading:10987503-Immunohistochemistry, pubmed-meshheading:10987503-Membrane Glycoproteins, pubmed-meshheading:10987503-Microscopy, Electron, pubmed-meshheading:10987503-Microscopy, Electron, Scanning, pubmed-meshheading:10987503-Necrosis, pubmed-meshheading:10987503-Neoplasm Transplantation, pubmed-meshheading:10987503-Phosphoric Diester Hydrolases, pubmed-meshheading:10987503-Platelet Aggregation, pubmed-meshheading:10987503-Protease Inhibitors, pubmed-meshheading:10987503-Proteoglycans, pubmed-meshheading:10987503-Rabbits, pubmed-meshheading:10987503-Sarcoma, Experimental, pubmed-meshheading:10987503-Serine Endopeptidases, pubmed-meshheading:10987503-Skin, pubmed-meshheading:10987503-Spider Venoms
pubmed:year
2000
pubmed:articleTitle
Effect of brown spider venom on basement membrane structures.
pubmed:affiliation
Department of Cell Biology, Federal University of Parana, Jardim das Americas, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't