Linked Life Data

10986562

Source:http://linkedlifedata.com/resource/pubmed/id/10986562

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2000-10-26
pubmed:abstractText
Dendritic cells (DCs) are potent antigen-presenting cells and are capable of activating naive T cells. Gene transfer of tumor antigen and cytokine genes into DCs could be an important strategy for immunotherapeutic applications. Dendritic cells derived from peripheral blood monocytes do not divide and are therefore poor candidates for gene transfer by Moloney murine leukemia virus (Mo-MuLV)-based retroviral vectors. Lentiviral vectors are emerging as a powerful tool for gene delivery into dividing and nondividing cells. A three-plasmid expression system pseudotyped with the envelope from vesicular stomatitis virus (VSV-G) was used to generate lentiviral vector particles expressing enhanced green fluorescent protein (EGFP). Peripheral blood monocyte-derived DCs were cultured in the presence of GM-CSF and IL-4 and transduced with lentiviral or Mo-MuLV-based vectors expressing EGFP. FACS analysis of lentiviral vector-transduced DCs derived either from normal healthy volunteers or from melanoma patients demonstrated transduction efficiency ranging from 70 to 90% compared with 2-8% using Mo-MuLV-based vectors pseudotyped with VSV-G. Comparison of lentiviral vectors expressing EGFP driven by CMV or human PGK promoters showed similar levels of transgene expression. Lentiviral vector preparations produced in the absence of HIV accessory proteins transduced DCs at efficiencies equal to vectors produced with accessory proteins. Alu-HIV-1 LTR PCR demonstrated the genomic integration of the lentiviral vector in the transduced DCs. Transduced cells showed characteristic dendritic cell phenotype and strong allostimulatory capacity and maintained the ability to respond to activation signals such as CD40 ligand and lipopolysaccharide. These results provide evidence that lentiviral vectors are efficient tools for gene transfer and expression in monocyte-derived DCs that could be useful for immunotherapeutic applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1043-0342
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1901-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10986562-Alu Elements, pubmed-meshheading:10986562-Antigens, CD, pubmed-meshheading:10986562-Cytomegalovirus, pubmed-meshheading:10986562-Dendritic Cells, pubmed-meshheading:10986562-Gene Transfer Techniques, pubmed-meshheading:10986562-Green Fluorescent Proteins, pubmed-meshheading:10986562-HIV-1, pubmed-meshheading:10986562-Humans, pubmed-meshheading:10986562-Immunoglobulins, pubmed-meshheading:10986562-Interleukin-12, pubmed-meshheading:10986562-Lentivirus, pubmed-meshheading:10986562-Luminescent Proteins, pubmed-meshheading:10986562-Melanoma, pubmed-meshheading:10986562-Membrane Glycoproteins, pubmed-meshheading:10986562-Moloney murine leukemia virus, pubmed-meshheading:10986562-Monocytes, pubmed-meshheading:10986562-Promoter Regions, Genetic, pubmed-meshheading:10986562-T-Lymphocytes, pubmed-meshheading:10986562-Vesicular stomatitis Indiana virus, pubmed-meshheading:10986562-Viral Proteins
pubmed:year
2000
pubmed:articleTitle
Efficient gene transfer to human peripheral blood monocyte-derived dendritic cells using human immunodeficiency virus type 1-based lentiviral vectors.
pubmed:affiliation
Clinical Gene Therapy Branch, NHGRI, Bethesda, MD 20892, USA.
pubmed:publicationType
Journal Article