Linked Life Data

10985772

Source:http://linkedlifedata.com/resource/pubmed/id/10985772

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
37
pubmed:dateCreated
2000-10-19
pubmed:abstractText
Thermodynamics of 66 RNA/DNA duplexes containing single mismatches were measured by UV melting methods. Stability enhancements for rG. dT mismatches were the largest of all mismatches examined here, while rU.dG mismatches were not as stable. The methyl group on C5 of thymine enhanced the stability by 0.12 approximately 0.53 kcal mol(-)(1) depending on the identity of adjacent Watson-Crick base pairs, whereas the 2'-hydroxyl group in ribouridine stabilized the duplex by approximately 0.6 kcal mol(-)(1) regardless of the adjacent base pairs. Stabilities induced by the methyl group in thymine, the 2'-hydroxyl group of ribouridine, and an nucleotide exchange at rG.dT and rU.dG mismatches were found to be independent of each other. The order for the mismatch stabilities is rG.dT >> rU. dG approximately rG.dG > rA.dG approximately rG.dA approximately rA. dC > rA.dA approximately rU.dT approximately rU.dC > rC.dA approximately rC.dT, although the identity of the adjacent base pairs slightly altered the order. The pH dependence stability and structural changes were suggested for the rA.dG but not for rG.dA mismatches. Comparisons of trinucleotide stabilities for G.T and G.U pairs in RNA, DNA, and RNA/DNA duplexes indicate that stable RNA/DNA mismatches exhibit a stability similar to RNA mismatches while unstable RNA/DNA mismatches show a stability similar to that of DNA mismatches. These results would be useful for the design of antisense oligonucleotides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11270-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10985772-Base Composition, pubmed-meshheading:10985772-Base Pair Mismatch, pubmed-meshheading:10985772-Circular Dichroism, pubmed-meshheading:10985772-DNA, pubmed-meshheading:10985772-Guanine, pubmed-meshheading:10985772-Guanosine, pubmed-meshheading:10985772-Hydrogen-Ion Concentration, pubmed-meshheading:10985772-Models, Molecular, pubmed-meshheading:10985772-Nucleic Acid Conformation, pubmed-meshheading:10985772-Nucleic Acid Heteroduplexes, pubmed-meshheading:10985772-Oligodeoxyribonucleotides, pubmed-meshheading:10985772-Oligoribonucleotides, pubmed-meshheading:10985772-RNA, pubmed-meshheading:10985772-Spectrophotometry, Ultraviolet, pubmed-meshheading:10985772-Structure-Activity Relationship, pubmed-meshheading:10985772-Thermodynamics, pubmed-meshheading:10985772-Thymidine, pubmed-meshheading:10985772-Uridine
pubmed:year
2000
pubmed:articleTitle
Thermodynamics-structure relationship of single mismatches in RNA/DNA duplexes.
pubmed:affiliation
Department of Chemistry, Faculty of Science, and High Technology Research Center, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan. sugimoto@konan-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't