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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1979-8-29
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pubmed:abstractText |
We have found the two-dimensional electrophoretic technique of O'Farrell to be highly efficient in the detection of charge-change substitutions in a large number of proteins. We have applied this method to determine the level of heterozygosity of the most abundant proteins in Drosophila melanogaster adults from a natural population. The estimate of per-locus heterozygosity obtained from approximately 54 loci screened was 4% with 6 loci polymorphic. This is much lower than overall estimates obtained by standard gel electrophoresis but is not different from estimates for "Group I" enzymes--i.e., those utilizing a narrow spectrum of substrates of intracellular origin. We consider these data to throw open the question of the level of genetic variability in nature.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-14158614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-14235730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-17248763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-191196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-265532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-271964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-349556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-357011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-413626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-4294340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-4379519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-4838763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-4847156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-5399253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-5968642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-5968643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-683345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-72513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109840-78766
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2381-4
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pubmed:dateRevised |
2010-9-1
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Reevaluation of level of genic heterozygosity in natural population of Drosophila melanogaster by two-dimensional electrophoresis.
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pubmed:publicationType |
Journal Article
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