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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2000-9-25
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pubmed:databankReference |
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pubmed:abstractText |
The proliferation disrupter (prod) gene of Drosophila melanogaster encodes a novel protein associated with centromeric chromosomal regions that is required for chromatin condensation and cell viability. We have examined the binding of the Prod protein to DNA in vitro. Co-immunoprecipitation experiments demonstrate that Prod is a DNA-binding protein that specifically recognizes the 10 bp AGAATAACAT satellite repeat of D.melanogaster. Footprinting experiments show that the protein interacts with a 5-8 bp target sequence in each 10 bp repeat and suggest that it can mediate condensation of this satellite into a superhelix. Gel retardation experiments indicate that Prod does not have a well defined DNA-binding domain and it binds the satellite in a co-operative manner, probably forming Prod multimers. Since Prod localizes to both heterochromatin and euchromatin in vivo, we discuss the possibility that the ability of pre-existing euchromatic proteins to bind DNA in a co-operative manner, might be a prerequisite of satellite compaction and satellite amplification, thereby providing a basic factor in heterochromatin evolution.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-1324929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-1385053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-1660135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-1779832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-2031185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-2110830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-3080746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-3118156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-3162306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-3960133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-4079979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-4084556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-4317352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-6260016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-6327070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-6818540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-7615663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-7813435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-7929083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-8063755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-8346553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-8375654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-8450539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-8620838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-8918191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-9009204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-9047333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-9352629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-9508764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-9613205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10982875-9927429
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3551-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10982875-Animals,
pubmed-meshheading:10982875-Binding Sites,
pubmed-meshheading:10982875-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:10982875-Cloning, Molecular,
pubmed-meshheading:10982875-DNA,
pubmed-meshheading:10982875-DNA-Binding Proteins,
pubmed-meshheading:10982875-Drosophila Proteins,
pubmed-meshheading:10982875-Drosophila melanogaster,
pubmed-meshheading:10982875-Heterochromatin,
pubmed-meshheading:10982875-Molecular Sequence Data,
pubmed-meshheading:10982875-Nucleic Acid Conformation,
pubmed-meshheading:10982875-Protein Binding,
pubmed-meshheading:10982875-Recombinant Fusion Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Prod is a novel DNA-binding protein that binds to the 1.686 g/cm(3) 10 bp satellite repeat of Drosophila melanogaster.
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pubmed:affiliation |
Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Szeged H-6701, Hungary. torok@nt.imp.univie.ac.at
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pubmed:publicationType |
Journal Article
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