| pubmed-article:10982790 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10982790 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10982790 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:10982790 | lifeskim:mentions | umls-concept:C2243049 | lld:lifeskim |
| pubmed-article:10982790 | lifeskim:mentions | umls-concept:C0039938 | lld:lifeskim |
| pubmed-article:10982790 | lifeskim:mentions | umls-concept:C0079189 | lld:lifeskim |
| pubmed-article:10982790 | lifeskim:mentions | umls-concept:C0035253 | lld:lifeskim |
| pubmed-article:10982790 | pubmed:issue | 48 | lld:pubmed |
| pubmed-article:10982790 | pubmed:dateCreated | 2000-12-18 | lld:pubmed |
| pubmed-article:10982790 | pubmed:abstractText | Human thioredoxin (Trx) catalyzes intracellular disulfide reductions but has also co-cytokine activity with interleukins after leaderless secretion. A recombinant truncated form of thioredoxin with the 80 N-terminal residues (Trx80) was purified to homogeneity. We discovered that Trx80 by itself is a potent mitogenic cytokine stimulating growth of resting human peripheral blood mononuclear cells. No effect was seen by Trx, but Trx80 at 50-100 nm induced cell proliferation of human peripheral blood mononuclear cells in serum-free synthetic medium, measured as [(3)H]thymidine incorporation after 72 h, with a maximum effect being comparable with that of 5 units/ml of interleukin-2. Trx80 lacked redox activity, but CD spectra suggested a secondary structure similar to Trx. Reduced Trx80 had an M(r) of 25,000, indicating that it is a dimer in solution. We also developed two different sandwich enzyme-linked immunosorbent assays that distinguish between full-length Trx and Trx80 and determined plasma levels of Trx and Trx80 in blood donors. The levels of Trx80 varied from 2 to 175 ng/ml; in comparison levels of Trx varied from 16 to 55 ng/ml without correlation to Trx80. In conclusion, the naturally occurring Trx80 is a novel mitogenic cytokine for normal resting human blood mononuclear cells. | lld:pubmed |
| pubmed-article:10982790 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10982790 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10982790 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10982790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10982790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10982790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10982790 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10982790 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10982790 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:10982790 | pubmed:author | pubmed-author:HolmgrenAA | lld:pubmed |
| pubmed-article:10982790 | pubmed:author | pubmed-author:GurunathRR | lld:pubmed |
| pubmed-article:10982790 | pubmed:author | pubmed-author:PekkariKK | lld:pubmed |
| pubmed-article:10982790 | pubmed:author | pubmed-author:ArnerE SES | lld:pubmed |
| pubmed-article:10982790 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10982790 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:10982790 | pubmed:volume | 275 | lld:pubmed |
| pubmed-article:10982790 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10982790 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10982790 | pubmed:pagination | 37474-80 | lld:pubmed |
| pubmed-article:10982790 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:10982790 | pubmed:meshHeading | pubmed-meshheading:10982790... | lld:pubmed |
| pubmed-article:10982790 | pubmed:meshHeading | pubmed-meshheading:10982790... | lld:pubmed |
| pubmed-article:10982790 | pubmed:meshHeading | pubmed-meshheading:10982790... | lld:pubmed |
| pubmed-article:10982790 | pubmed:meshHeading | pubmed-meshheading:10982790... | lld:pubmed |
| pubmed-article:10982790 | pubmed:meshHeading | pubmed-meshheading:10982790... | lld:pubmed |
| pubmed-article:10982790 | pubmed:meshHeading | pubmed-meshheading:10982790... | lld:pubmed |
| pubmed-article:10982790 | pubmed:meshHeading | pubmed-meshheading:10982790... | lld:pubmed |
| pubmed-article:10982790 | pubmed:meshHeading | pubmed-meshheading:10982790... | lld:pubmed |
| pubmed-article:10982790 | pubmed:meshHeading | pubmed-meshheading:10982790... | lld:pubmed |
| pubmed-article:10982790 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10982790 | pubmed:articleTitle | Truncated thioredoxin is a mitogenic cytokine for resting human peripheral blood mononuclear cells and is present in human plasma. | lld:pubmed |
| pubmed-article:10982790 | pubmed:affiliation | Medical Nobel Institute for Biochemistry, Department of Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden. | lld:pubmed |
| pubmed-article:10982790 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10982790 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:7295 | entrezgene:pubmed | pubmed-article:10982790 | lld:entrezgene |
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