Source:http://linkedlifedata.com/resource/pubmed/id/10980012
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2000-10-3
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| pubmed:abstractText |
Whole cell patch-clamp recordings using Cl(-)-filled pipettes revealed more negative levels of baseline current and associated current variance in embryonic rat hippocampal neurons co-cultured on a monolayer of astrocytes than those cultured on poly-D-lysine. These effects were mimicked by culturing neurons on poly-D-lysine in astrocyte-conditioned medium (ACM). The baseline current and variance decreased immediately in all cells after either local perfusion with saline or exposure to bicuculline, an antagonist of GABA at GABA(A) receptor/Cl(-) channels. Baseline current and variance in all cells reached a nadir at approximately 0 mV, the calculated equilibrium potential for Cl(-). Perfusion of ACM rapidly induced a sustained current in neurons, which also reversed polarity at approximately 0 mV. Bicuculline attenuated or eliminated the ACM-induced current at a concentration that completely blocked micromolar GABA-induced current. Quantitative analyses of spontaneously occurring fluctuations superimposed on the ACM-induced current revealed estimated unitary properties of the underlying channel activity similar to those calculated for GABA's activation of GABA(A) receptor/Cl(-) channels. Bicuculline-sensitive synaptic-like transients, which reversed at approximately 0 mV, were also detected in neurons cultured in ACM, and these were immediately eliminated along with the negative baseline current and superimposed current fluctuations by perfusion. Furthermore bicuculline-sensitive synaptic-like transients were rapidly and reversibly triggered when ACM was acutely applied. ACM induced an increase in cytoplasmic Ca(2+) in cultured embryonic hippocampal neurons that was completely blocked by bicuculline and strychnine. We conclude that astrocytes release diffusible substances, most likely GABA, that persistently activate GABA(A) receptor/Cl(-) channels in co-cultured neurons.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-bis(2-aminophenoxy)ethane...,
http://linkedlifedata.com/resource/pubmed/chemical/3-Mercaptopropionic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Bicuculline,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/GABA Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/GABA-A Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, GABA-A,
http://linkedlifedata.com/resource/pubmed/chemical/Strychnine,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Aminobutyric Acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0022-3077
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
84
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1392-403
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:10980012-3-Mercaptopropionic Acid,
pubmed-meshheading:10980012-Animals,
pubmed-meshheading:10980012-Animals, Newborn,
pubmed-meshheading:10980012-Astrocytes,
pubmed-meshheading:10980012-Bicuculline,
pubmed-meshheading:10980012-Calcium,
pubmed-meshheading:10980012-Cell Differentiation,
pubmed-meshheading:10980012-Cells, Cultured,
pubmed-meshheading:10980012-Chloride Channels,
pubmed-meshheading:10980012-Chlorides,
pubmed-meshheading:10980012-Coculture Techniques,
pubmed-meshheading:10980012-Culture Media, Conditioned,
pubmed-meshheading:10980012-Egtazic Acid,
pubmed-meshheading:10980012-GABA Antagonists,
pubmed-meshheading:10980012-GABA-A Receptor Antagonists,
pubmed-meshheading:10980012-Glycine Agents,
pubmed-meshheading:10980012-Hippocampus,
pubmed-meshheading:10980012-Membrane Potentials,
pubmed-meshheading:10980012-Neurons,
pubmed-meshheading:10980012-Patch-Clamp Techniques,
pubmed-meshheading:10980012-Rats,
pubmed-meshheading:10980012-Receptors, GABA-A,
pubmed-meshheading:10980012-Strychnine,
pubmed-meshheading:10980012-gamma-Aminobutyric Acid
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| pubmed:year |
2000
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| pubmed:articleTitle |
Persistent activation of GABA(A) receptor/Cl(-) channels by astrocyte-derived GABA in cultured embryonic rat hippocampal neurons.
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| pubmed:affiliation |
Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-4066, USA.
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| pubmed:publicationType |
Journal Article
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