Linked Life Data

10979185

Source:http://linkedlifedata.com/resource/pubmed/id/10979185

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-9-25
pubmed:abstractText
The region between RFLP markers G342 and R1167 was the subterminal part of the longer arm on the rice chromosome 6, because Shen et al. (1998) mapped two telomeric repeat associated sequences distal to G342. In order to integrate the genetic map and the physical map of the region, G342 and R1167 were firstly used to screen BAC library. Based on the positive clones detected by the two markers and chromosome walking by using the outer most insert-end of the overlapping clones, a contig containing 16 BAC clones which spanned 500 kb was constructed. All the insert-ends of the BAC clones could be amplified with thermal asymmetric interlaced PCR. Fourteen insert-ends were subcloned. Seven of them were identified as a single or low copy sequences and five were mapped on the expected sites flanking G342 or R1167. The insert fragment isolated from the minimum tile BAC clones of the contig was used to screen a cDNA library and four different positive clones were detected.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0379-4172
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
400-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
[Integration of the genetic map and the physical map of the subterminal region on the longer arm of rice chromosome 6].
pubmed:affiliation
Institute of Genetics, Chinese Academy of Sciences, Beijing, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't