Source:http://linkedlifedata.com/resource/pubmed/id/10978740
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2-3
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pubmed:dateCreated |
2001-1-22
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pubmed:abstractText |
Three full-length cDNAs, one encoding the precursor of a beta-toxin-like peptide (named BmKBT) and the other two encoding those of (MkTx I) homologues (named MkTx II and MkTx III, respectively), were isolated from a venom gland cDNA library of the Chinese scorpion Buthus martensii Karsch, by screening with a cDNA fragment generated by PCR. The encoded precursor of BmKBT contained 83 amino acid residues including a signal peptide of 19 residues, a mature peptide of 63 residues and an extra basic residue (Lys) which have to be removed in the processing step. The deduced amino acid sequence of BmKBT showed 52% homology to that of beta-neurotoxin TsVII isolated from scorpion Tityus serrulatus. However, the positions of disulfide bridges have a little variation between the two peptides. The precursors of MkTx II and MkTx III both contained 85 amino acid residues including a signal peptide of 19 residues, a mature peptide of 64 residues and two extra residues (Gly-Arg) which have to be removed in the processing step, too. There was high sequence similarity (90%) between the two peptides. The sequences of mature MkTx II and MkTx III were highly homologous with MkTx I isolated from scorpion Buthus martensii Karsch, both showing 90% identities.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Scorpion Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/TX1 toxin, Buthus,
http://linkedlifedata.com/resource/pubmed/chemical/TX2 toxin, Buthus
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pubmed:status |
MEDLINE
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pubmed:issn |
0041-0101
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
225-32
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10978740-Amino Acid Sequence,
pubmed-meshheading:10978740-Animals,
pubmed-meshheading:10978740-Base Sequence,
pubmed-meshheading:10978740-Cloning, Molecular,
pubmed-meshheading:10978740-DNA, Complementary,
pubmed-meshheading:10978740-Gene Library,
pubmed-meshheading:10978740-Molecular Sequence Data,
pubmed-meshheading:10978740-Neurotoxins,
pubmed-meshheading:10978740-Polymerase Chain Reaction,
pubmed-meshheading:10978740-Scorpion Venoms,
pubmed-meshheading:10978740-Scorpions,
pubmed-meshheading:10978740-Sequence Analysis, DNA
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pubmed:articleTitle |
Molecular cloning and sequence analysis of cDNAs encoding a beta-toxin-like peptide and two MkTx I homologues from scorpion Buthus martensii Karsch.
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pubmed:affiliation |
Department of Biotechnology, College of Life Sciences, Institute of Virology, Wuhan University, Wuhan 430072, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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