Linked Life Data

10978145

Source:http://linkedlifedata.com/resource/pubmed/id/10978145

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
35
pubmed:dateCreated
2000-9-28
pubmed:abstractText
The low-density lipoprotein receptor-related protein (LRP) is a large surface receptor that mediates binding and internalization of a large number of structurally and functionally unrelated ligands. The ligand binding sites are located in clusters of complement-type repeats (CR), where the general absence of mutual binding competition suggests that different ligands map to distinct sites. Binding of alpha(2)-macroglobulin-protease complexes to the LRP is mediated by the receptor binding domain (RBD) of alpha(2)-macroglobulin (alpha(2)M). To determine the major binding epitope(s) in the LRP, we generated a complete set of tandem CR proteins spanning the second cluster of CR domains, and identified a binding site for alpha(2)M in the N-terminal part of the cluster comprising CR3-CR6, using ligand blotting and surface plasmon resonance (SPR) analysis. The specific site involved in alpha(2)M recognition resides in the fourth CR domain, CR4, whereas another site is identified in CR5. An acidic epitope in CR4 is identified as important for binding alpha(2)M by mutagenesis and SPR analysis. The formation of the complex between the rat alpha(1)-macroglobulin RBD and CR domain pairs is characterized by analytical size-exclusion chromatography, which demonstrates a sufficiently strong interaction between the alpha(1)M RBD and CR34 or CR45 for the isolation of a complex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10627-33
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:10978145-Amino Acid Sequence, pubmed-meshheading:10978145-Animals, pubmed-meshheading:10978145-Complement System Proteins, pubmed-meshheading:10978145-Epidermal Growth Factor, pubmed-meshheading:10978145-Heymann Nephritis Antigenic Complex, pubmed-meshheading:10978145-Humans, pubmed-meshheading:10978145-Low Density Lipoprotein Receptor-Related Protein-1, pubmed-meshheading:10978145-Membrane Glycoproteins, pubmed-meshheading:10978145-Models, Molecular, pubmed-meshheading:10978145-Molecular Sequence Data, pubmed-meshheading:10978145-Peptide Fragments, pubmed-meshheading:10978145-Protein Binding, pubmed-meshheading:10978145-Protein Structure, Tertiary, pubmed-meshheading:10978145-Rats, pubmed-meshheading:10978145-Receptors, Immunologic, pubmed-meshheading:10978145-Receptors, LDL, pubmed-meshheading:10978145-Recombinant Fusion Proteins, pubmed-meshheading:10978145-Repetitive Sequences, Amino Acid, pubmed-meshheading:10978145-alpha-Macroglobulins
pubmed:year
2000
pubmed:articleTitle
Specific binding of alpha-macroglobulin to complement-type repeat CR4 of the low-density lipoprotein receptor-related protein.
pubmed:affiliation
Laboratory of Gene Expression, Department of Molecular and Structural Biology, and Department of Medical Biochemistry, University of Aarhus, Aarhus, Denmark. hct@biobase.dk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't