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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2000-10-10
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pubmed:abstractText |
The regulation of the C/EBP family in macrophages by LPS and cytokines is of potentially crucial importance in several pathophysiological conditions. The action of LPS and three cytokines on the expression of C/EBP mRNA, protein and functional DNA binding activity in the murine J774.2 cell line was therefore studied. Exposure of the cells to LPS, IL-1, IFN-gamma and TNF-alpha produced a reduction of C/EBP alpha mRNA levels and a corresponding increase in the expression of C/EBP beta and C/EBP delta. EMSA showed time-dependent changes in the DNA binding activity of individual C/EBP isoforms and demonstrated the participation of heterodimers between the different members in DNA-protein interactions. Additionally, mediator-specific changes in the kinetics and magnitude of C/EBP mRNA expression pattern and profile of DNA-protein interactions were observed. These studies provide novel insights into the potential mechanisms that may be responsible for the mediator-specific regulation of macrophage gene expression through the C/EBP family.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1430-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10976009-Animals,
pubmed-meshheading:10976009-Blotting, Western,
pubmed-meshheading:10976009-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:10976009-Cell Line,
pubmed-meshheading:10976009-Cell Nucleus,
pubmed-meshheading:10976009-Cytokines,
pubmed-meshheading:10976009-DNA,
pubmed-meshheading:10976009-Interferon-gamma,
pubmed-meshheading:10976009-Interleukin-1,
pubmed-meshheading:10976009-Kinetics,
pubmed-meshheading:10976009-Lipopolysaccharides,
pubmed-meshheading:10976009-Macrophages,
pubmed-meshheading:10976009-Mice,
pubmed-meshheading:10976009-Protein Binding,
pubmed-meshheading:10976009-Protein Isoforms,
pubmed-meshheading:10976009-RNA, Messenger,
pubmed-meshheading:10976009-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10976009-Time Factors,
pubmed-meshheading:10976009-Tumor Necrosis Factor-alpha
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pubmed:year |
2000
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pubmed:articleTitle |
Differential regulation of macrophage CCAAT-enhancer binding protein isoforms by lipopolysaccharide and cytokines.
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pubmed:affiliation |
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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