Source:http://linkedlifedata.com/resource/pubmed/id/10975862
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2000-10-10
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pubmed:abstractText |
Chemokines and their receptors determine the distribution of leukocytes within tissues in health and disease. We have studied the role of the constitutive chemokine receptor CXCR4 and its ligand, stromal-derived factor-1 (SDF-1) in the perivascular accumulation of T cells in rheumatoid arthritis. We show that synovial T cells, which are primed CD45RO+CD45RBdull cells and consequently not expected to express constitutive chemokine receptors, have high levels of the chemokine receptor CXCR4. Sustained expression of CXCR4 was maintained on synovial T cells by specific factors present within the synovial microenvironment. Extensive screening revealed that TGF-beta isoforms induce the expression of CXCR4 on CD4 T cells in vitro. Depletion studies using synovial fluid confirmed an important role for TGF-beta1 in the induction of CXCR4 expression in vivo. The only known ligand for CXCR4 is SDF-1. We found SDF-1 on synovial endothelial cells and showed that SDF-1 was able to induce strong integrin-mediated adhesion of synovial fluid T cells to fibronectin and ICAM-1, confirming that CXCR4 expressed on synovial T cells was functional. These results suggest that the persistent induction of CXCR4 on synovial T cells by TGF-beta1 leads to their active, SDF-1-mediated retention in a perivascular distribution within the rheumatoid synovium.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CXCL12 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR4,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3423-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10975862-Arthritis, Rheumatoid,
pubmed-meshheading:10975862-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10975862-Cell Differentiation,
pubmed-meshheading:10975862-Cell Line,
pubmed-meshheading:10975862-Cell Movement,
pubmed-meshheading:10975862-Cells, Cultured,
pubmed-meshheading:10975862-Chemokine CXCL12,
pubmed-meshheading:10975862-Chemokines, CXC,
pubmed-meshheading:10975862-Endothelium, Vascular,
pubmed-meshheading:10975862-Humans,
pubmed-meshheading:10975862-Lymphocyte Activation,
pubmed-meshheading:10975862-Receptors, CXCR4,
pubmed-meshheading:10975862-Stromal Cells,
pubmed-meshheading:10975862-Synovial Fluid,
pubmed-meshheading:10975862-Synovial Membrane,
pubmed-meshheading:10975862-T-Lymphocyte Subsets,
pubmed-meshheading:10975862-Transforming Growth Factor beta
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pubmed:year |
2000
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pubmed:articleTitle |
Persistent induction of the chemokine receptor CXCR4 by TGF-beta 1 on synovial T cells contributes to their accumulation within the rheumatoid synovium.
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pubmed:affiliation |
Division of Immunity and Infection, Medical Research Council Center for Immune Regulation, University of Birmingham, Edgbaston, United Kingdom. c.d.buckley@bham.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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