Source:http://linkedlifedata.com/resource/pubmed/id/10975466
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2000-9-18
|
| pubmed:databankReference | |
| pubmed:abstractText |
The human alcohol dehydrogenase gene ADH6 is expressed at the highest levels in fetal and adult liver. We have mapped cis-acting elements that affect its expression. The sequence from bp -34 to -62 (site C) that includes the TATA box was strongly bound by nuclear proteins from liver, hepatoma cells, and fibroblasts. A truncation that removed the upstream part of site C but left the TATA homology intact dramatically reduced transcription; altering 5 bp in this region had much less effect. Part of site C can be bound by C/EBPalpha, but cotransfection with C/EBPalpha or C/EBPbeta did not stimulate transcription. The proximal region did not display tissue specificity, so we cloned the upstream region to search for additional regulatory sequences. The region between -1.6 and -2.3 kb stimulated transcription in hepatoma cells and inhibited it in fibroblasts. We identified two sites in this region that affect transcription independently of their orientation. Site 1 was a negative regulatory element in fibroblasts but had no effect in hepatoma cells. Site 2 was a positive regulatory element in hepatoma cells but had no effect in fibroblasts. This combination of positive and negative regulatory elements can play a significant role in the tissue-specific expression of ADH6.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1044-5498
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
487-97
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:10975466-Alcohol Dehydrogenase,
pubmed-meshheading:10975466-Base Sequence,
pubmed-meshheading:10975466-Binding Sites,
pubmed-meshheading:10975466-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:10975466-DNA Footprinting,
pubmed-meshheading:10975466-DNA-Binding Proteins,
pubmed-meshheading:10975466-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10975466-Genomic Library,
pubmed-meshheading:10975466-Humans,
pubmed-meshheading:10975466-Molecular Sequence Data,
pubmed-meshheading:10975466-Multigene Family,
pubmed-meshheading:10975466-Mutagenesis, Site-Directed,
pubmed-meshheading:10975466-Nuclear Proteins,
pubmed-meshheading:10975466-Promoter Regions, Genetic,
pubmed-meshheading:10975466-Protein Binding,
pubmed-meshheading:10975466-Tissue Distribution,
pubmed-meshheading:10975466-Transcription, Genetic
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Tissue-specific regulatory elements in the human alcohol dehydrogenase 6 gene.
|
| pubmed:affiliation |
Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis 46202-5122, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|