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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1975-11-6
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pubmed:abstractText |
Different dominant thermosensitive mutations affecting the same gene were selected in Saccharomyces cerevisiae. Ribonucleic acid (RNA) synthesis decreased rapidly and markedly at 37 C in all the mutants whether they were in a homozygous or a heterozygous state. Protein biosynthesis was at first unaffected and then decreased slowly, stopping after 5 h. Measurements of RNA biosynthesis in isolated nuclei as well as in vitro activities of RNA polymerases A and B at 22 and 37 C failed to reveal any difference between mutants and the wild type. Analysis of the nature of the residual RNAs synthesized at the high temperature in the mutants showed a small relative increase in the messenger RNA fraction, but it was not sufficient to indicate a specific inactivation of RNA polymerase A activity. The results suggest an impairment in a common regulatory element for all RNA polymerases acting at the level of the initiation of transcription. Similar mutants with a semidominant phenotype were obtained in which the lesions were in two other unlinked loci.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-13473180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4108111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4370349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4525293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4557192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4558656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4570761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4573680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4582157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4583213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4591362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4606699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-4790623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-5267147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-5337848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-5488085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-5573734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-5900603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-5965527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1097402-6066050
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
122
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
847-54
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1097402-Antifungal Agents,
pubmed-meshheading:1097402-Cell Nucleus,
pubmed-meshheading:1097402-Cell-Free System,
pubmed-meshheading:1097402-Cycloheximide,
pubmed-meshheading:1097402-DNA-Directed RNA Polymerases,
pubmed-meshheading:1097402-Fungal Proteins,
pubmed-meshheading:1097402-Genes, Dominant,
pubmed-meshheading:1097402-Isoenzymes,
pubmed-meshheading:1097402-Mutation,
pubmed-meshheading:1097402-Nitrates,
pubmed-meshheading:1097402-Phenazines,
pubmed-meshheading:1097402-Phenotype,
pubmed-meshheading:1097402-Pyrroles,
pubmed-meshheading:1097402-RNA,
pubmed-meshheading:1097402-Saccharomyces cerevisiae,
pubmed-meshheading:1097402-Temperature,
pubmed-meshheading:1097402-Transcription, Genetic
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pubmed:year |
1975
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pubmed:articleTitle |
Dominant and semidominant mutations leading to thermosensitivity of ribonucleic acid biosynthesis in Saccharomyces cerevisiae.
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pubmed:publicationType |
Journal Article
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