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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0018270,
umls-concept:C0023837,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0162508,
umls-concept:C0205245,
umls-concept:C0221920,
umls-concept:C1335858,
umls-concept:C1521761,
umls-concept:C1704675
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pubmed:issue |
19
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pubmed:dateCreated |
2000-10-12
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pubmed:abstractText |
The functional role of the interaction between c-Jun and simian virus 40 promoter factor 1 (Sp1) in epidermal growth factor (EGF)-induced expression of 12(S)-lipoxygenase gene in human epidermoid carcinoma A431 cells was studied. Coimmunoprecipitation experiments indicated that EGF stimulated interaction between c-Jun and Sp1 in a time-dependent manner. Overexpression of Ha-ras and c-Jun also enhanced the amount of c-Jun binding to Sp1. In addition, the c-Jun dominant negative mutant TAM-67 not only inhibited the coimmunoprecipitated c-Jun binding to Sp1 in a dose-dependent manner in cells overexpressing c-Jun but also reduced promoter activity of the 12(S)-lipoxygenase gene induced by c-Jun overexpression. Treatment of cells with EGF increased the interaction between the Sp1 oligonucleotide and nuclear c-Jun/Sp1 in a time-dependent manner. Furthermore, EGF activated the chimeric promoter consisting of 10 tandem GAL4-binding sites, which replaced the three Sp1-binding sites in the 12(S)lipoxygenase promoter only when coexpressed with GAL4-c-Jun () fusion proteins. These results indicate that the direct interaction between c-Jun and Sp1 induced by EGF cooperatively activated expression of the 12(S)-lipoxygenase gene, and that Sp1 may serve at least in part as a carrier bringing c-Jun to the promoter, thus transactivating the transcriptional activity of 12(S)-lipoxygenase gene.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-10391891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-10428482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-10441514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-10506225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-1061097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-10617690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-1447217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-1454785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-1516134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-1740418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-1749429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-1846781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-1903181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-1922387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-2133107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-2217179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-2377602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-2916128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-2995499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-3018088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-3805758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-4215079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-6807297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-7525931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-7739528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-7935378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-8304420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-8327494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-8344930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-8360167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-8493579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-9022753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-9059517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-9069263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-9164849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-9254887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-9443600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10973489-9837920
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 12-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GAL4 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sp1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10406-11
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10973489-Arachidonate 12-Lipoxygenase,
pubmed-meshheading:10973489-Base Sequence,
pubmed-meshheading:10973489-DNA Primers,
pubmed-meshheading:10973489-DNA-Binding Proteins,
pubmed-meshheading:10973489-Epidermal Growth Factor,
pubmed-meshheading:10973489-Fungal Proteins,
pubmed-meshheading:10973489-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10973489-Genes, ras,
pubmed-meshheading:10973489-Humans,
pubmed-meshheading:10973489-Precipitin Tests,
pubmed-meshheading:10973489-Promoter Regions, Genetic,
pubmed-meshheading:10973489-Protein Binding,
pubmed-meshheading:10973489-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:10973489-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:10973489-Sp1 Transcription Factor,
pubmed-meshheading:10973489-Tetradecanoylphorbol Acetate,
pubmed-meshheading:10973489-Transcription Factors,
pubmed-meshheading:10973489-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
Functional interaction between c-Jun and promoter factor Sp1 in epidermal growth factor-induced gene expression of human 12(S)-lipoxygenase.
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pubmed:affiliation |
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
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