Linked Life Data

10972984

Source:http://linkedlifedata.com/resource/pubmed/id/10972984

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-1-5
pubmed:abstractText
The nuclear matrix-intermediate filament complex (NM-IF) is a protein scaffold which spans the whole cell, and several lines of evidence suggest that this structural frame represents also a functional unit, which could be involved in the epigenetic control of cancer development. Here we report the characterization by high resolution two-dimensional gel electrophoresis and Western blot analysis of the NM-IF complex isolated from prostate cancer (PCa); tumor-associated proteins were identified by comparing the electrophoretic patterns with those of normal human prostate (NHP). Extensive changes in the expression of both the NM and IF proteins occur; they are, however, related in a different way to tumor progression. Poorly differentiated PCa (Gleason score 8-9) shows a strong down regulation of several constitutive cytokeratins (CKs 8, 18, and 19); their expression significantly (P < 0.05) decreases with respect to both NHP and benign prostatic hyperplasia (BPH) and, more interestingly, also with respect to moderately (Gleason score 6-7) and well (Gleason score 4-5) differentiated tumors. Moreover, we have identified a tumor-associated species which is present in all of the tumors examined, systematically absent in NHP and occurs only in a few samples of BPH; this polypeptide, of M(r) 48,000 and pI 6.0, represent a proteolytic fragment of CK8. At variance with these continuing alterations in the expression, the NM proteins undergo stepwise changes correlating with the level of differentiation. The development of less differentiated tumors is characterized by the appearance of several new proteins and by the decrease in the expression of others. Six proteins were found to be expressed with a frequency equal to one in poorly differentiated tumor, namely in all the samples of tumor examined, while in moderately and well differentiated tumors the frequency is less than one, and decreases with increasing the level of differentiation. When tumors of increasing Gleason score are compared with NHP a dramatic increase in the complexity of the protein patterns is observed, indicating that tumor dedifferentiation results in a considerable increase in the phenotypic diversity. These results suggest that tumor progression can be characterized using an appropriate subset of tumor-associated NM proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0730-2312
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
471-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10972984-Adenocarcinoma, pubmed-meshheading:10972984-Aged, pubmed-meshheading:10972984-Antigens, Nuclear, pubmed-meshheading:10972984-Cell Differentiation, pubmed-meshheading:10972984-Disease Progression, pubmed-meshheading:10972984-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:10972984-Gene Expression Regulation, Neoplastic, pubmed-meshheading:10972984-Humans, pubmed-meshheading:10972984-Isoelectric Focusing, pubmed-meshheading:10972984-Keratins, pubmed-meshheading:10972984-Macromolecular Substances, pubmed-meshheading:10972984-Male, pubmed-meshheading:10972984-Middle Aged, pubmed-meshheading:10972984-Neoplasm Proteins, pubmed-meshheading:10972984-Nuclear Proteins, pubmed-meshheading:10972984-Phenotype, pubmed-meshheading:10972984-Prostatic Hyperplasia, pubmed-meshheading:10972984-Prostatic Neoplasms, pubmed-meshheading:10972984-Protein Isoforms, pubmed-meshheading:10972984-Subtraction Technique
pubmed:year
2000
pubmed:articleTitle
Changes in the expression of cytokeratins and nuclear matrix proteins are correlated with the level of differentiation in human prostate cancer.
pubmed:affiliation
Istituto Nazionale per la Ricerca sul Cancro, I-16132 Genova, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't