|
rdf:type |
|
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lifeskim:mentions |
umls-concept:C0004358,
umls-concept:C0014441,
umls-concept:C0020846,
umls-concept:C0020852,
umls-concept:C0025663,
umls-concept:C0205369,
umls-concept:C0229671,
umls-concept:C0439851,
umls-concept:C0679932,
umls-concept:C1552596,
umls-concept:C1947931,
umls-concept:C2346501
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|
pubmed:issue |
4
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|
pubmed:dateCreated |
2000-10-13
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|
pubmed:abstractText |
In measuring specific IgE levels in sera by direct ELISA, competition with coexisting IgG often impedes an exact IgE determination; additionally, IgG autoantibodies to IgE (IgG-IgE) in sera affect the assay. In this paper, we attempt to determine accurate specific IgE levels by selective removal of IgG with a protein G-immobilized gel (PG) and by acid treatment of the PG to compensate for the unintended removal of IgE, probably due to the PG binding IgG-IgE.
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pubmed:language |
eng
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pubmed:journal |
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|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1018-2438
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|
pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 S. Karger AG, Basel.
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pubmed:issnType |
Print
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pubmed:volume |
122
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pubmed:owner |
NLM
|
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pubmed:authorsComplete |
Y
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pubmed:pagination |
264-9
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:10971117-Animals,
pubmed-meshheading:10971117-Autoantibodies,
pubmed-meshheading:10971117-Bacterial Proteins,
pubmed-meshheading:10971117-Binding, Competitive,
pubmed-meshheading:10971117-Cattle,
pubmed-meshheading:10971117-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10971117-Humans,
pubmed-meshheading:10971117-Hydrogen-Ion Concentration,
pubmed-meshheading:10971117-Immunoglobulin E,
pubmed-meshheading:10971117-Immunoglobulin G,
pubmed-meshheading:10971117-Milk Hypersensitivity,
pubmed-meshheading:10971117-Radioallergosorbent Test
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|
pubmed:year |
2000
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|
pubmed:articleTitle |
A method for measuring specific IgE in sera by direct ELISA without interference by IgG competition or IgG autoantibodies to IgE.
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|
pubmed:affiliation |
Nutritional Science Laboratory, Snow Brand Milk Products Co. Ltd., Kawagoe, Saitama, Japan.
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pubmed:publicationType |
Journal Article
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