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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-11-29
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pubmed:abstractText |
The roles of P(2X1)and P(2T AC)receptors in ADP-evoked Ca(2+)signalling were investigated in fura-2-loaded human platelets. Desensitization of the P(2X1)receptor with the selective agonist, alphabeta-methylene ATP, reduced the integral of the ADP-evoked rise in [Ca(2+)](i)to about 90% of control; a reduction equivalent to the integral of the P(2X1)-evoked response alone. After elevating cAMP or cGMP levels using prostaglandin E(1)or sodium nitroprusside, prior P(2X1)desensitization reduced the integral of the ADP-evoked response to about 70% of control. This reduction was greater than the integral of the P(2X1)-evoked response alone under the same conditions, suggesting rapidly activated Ca(2+)entry via the P(2X1)receptor potentiates Ca(2+)responses evoked via the phospholipase C-coupled P(2Y1)receptor. The P(2T AC)receptor antagonist, AR-C69931MX, at a concentration completely inhibiting aggregation, did not significantly affect the initial peaks but caused a significant reduction in the integrals of the ADP-evoked rises in [Ca(2+)](i)to about 71% or 77% of controls in the presence or absence of external Ca(2+)respectively. This suggests that the main effect of lowering cAMP levels after inhibition of adenylyl cyclase via P(2T AC)receptors may be reduced Ca(2+)removal from the cytosol. These results indicate that both the P(2X1)and P(2T AC)receptors play a significant role in ADP-evoked Ca(2+)signalling in human platelets.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Monophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/P2RY12 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Aggregation Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic P2 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic P2 Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2X,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2Y12,
http://linkedlifedata.com/resource/pubmed/chemical/alpha,beta-methyleneadenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/cangrelor
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0143-4160
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
119-26
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10970768-Adenosine Diphosphate,
pubmed-meshheading:10970768-Adenosine Monophosphate,
pubmed-meshheading:10970768-Adenosine Triphosphate,
pubmed-meshheading:10970768-Blood Platelets,
pubmed-meshheading:10970768-Calcium Signaling,
pubmed-meshheading:10970768-Humans,
pubmed-meshheading:10970768-Membrane Proteins,
pubmed-meshheading:10970768-Platelet Aggregation Inhibitors,
pubmed-meshheading:10970768-Purinergic P2 Receptor Agonists,
pubmed-meshheading:10970768-Purinergic P2 Receptor Antagonists,
pubmed-meshheading:10970768-Receptors, Purinergic P2,
pubmed-meshheading:10970768-Receptors, Purinergic P2X,
pubmed-meshheading:10970768-Receptors, Purinergic P2Y12
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pubmed:year |
2000
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pubmed:articleTitle |
The roles of P(2X1)and P(2T AC)receptors in ADP-evoked calcium signalling in human platelets.
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pubmed:affiliation |
Department of Physiology, University of Cambridge, Cambridge, UK. sos10@cam.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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