|
rdf:type |
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|
lifeskim:mentions |
|
|
pubmed:issue |
16
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|
pubmed:dateCreated |
2000-12-6
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|
pubmed:abstractText |
A 16-residue peptide, including the Tat(49-57) sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA.
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|
pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0960-894X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
21
|
|
pubmed:volume |
10
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1857-61
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|
pubmed:dateRevised |
2006-11-15
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|
pubmed:meshHeading |
pubmed-meshheading:10969985-Drug Evaluation, Preclinical,
pubmed-meshheading:10969985-Fluoresceins,
pubmed-meshheading:10969985-Fluorescent Dyes,
pubmed-meshheading:10969985-Gene Products, tat,
pubmed-meshheading:10969985-HIV Long Terminal Repeat,
pubmed-meshheading:10969985-Humans,
pubmed-meshheading:10969985-Molecular Structure,
pubmed-meshheading:10969985-Peptide Fragments,
pubmed-meshheading:10969985-Peptides,
pubmed-meshheading:10969985-Spectrometry, Fluorescence
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|
pubmed:year |
2000
|
|
pubmed:articleTitle |
A high-throughput screening utilizing intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically.
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|
pubmed:affiliation |
Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
