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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2000-9-27
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pubmed:abstractText |
The human opioid receptor-like (ORL(1)) receptor was tagged with the immunoglobulin G1 Fc fragment at the carboxy-terminus and expressed in human embryonic kidney 293 cells. Expression of the ORL(1)-Fc receptor was confirmed by immunohistochemical staining. The fusion protein was enriched by affinity chromatography and then verified by immunodetection. The function of the ORL(1)-Fc receptor was determined by examining nociceptin/OFQ-induced inhibition of cAMP accumulation. The ORL(1)-Fc receptor inhibited the forskolin-stimulated cAMP accumulation. The inhibitory response was selectively induced by nociceptin/OFQ in a dose-dependent and pertussis toxin-sensitive manner. Our results indicate that the carboxy-terminal Fc-tagged ORL(1) receptor retained the ability to interact with G(i) proteins to inhibit adenylyl cyclase.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Opioid Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fc,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella,
http://linkedlifedata.com/resource/pubmed/chemical/nociceptin,
http://linkedlifedata.com/resource/pubmed/chemical/nociceptin receptor
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pubmed:status |
MEDLINE
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pubmed:issn |
1422-4933
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 S. Karger AG, Basel.
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
240-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10965058-Adenylate Cyclase,
pubmed-meshheading:10965058-Adenylate Cyclase Toxin,
pubmed-meshheading:10965058-Cell Line,
pubmed-meshheading:10965058-Cyclic AMP,
pubmed-meshheading:10965058-Dose-Response Relationship, Drug,
pubmed-meshheading:10965058-Forskolin,
pubmed-meshheading:10965058-GTP-Binding Proteins,
pubmed-meshheading:10965058-Humans,
pubmed-meshheading:10965058-Immunoglobulin G,
pubmed-meshheading:10965058-Opioid Peptides,
pubmed-meshheading:10965058-Pertussis Toxin,
pubmed-meshheading:10965058-Receptors, Fc,
pubmed-meshheading:10965058-Receptors, Opioid,
pubmed-meshheading:10965058-Sequence Tagged Sites,
pubmed-meshheading:10965058-Virulence Factors, Bordetella
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pubmed:articleTitle |
Immunoglobulin G1 Fc fragment-tagged human opioid receptor-like receptor retains the ability to inhibit cAMP accumulation.
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pubmed:affiliation |
Department of Biology and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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