Linked Life Data

10964944

Source:http://linkedlifedata.com/resource/pubmed/id/10964944

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2000-9-28
pubmed:abstractText
The CaMKIIalpha mRNA extends into distal hippocampal dendrites, and the 3' untranslated region (3'UTR) is sufficient to mediate this localization. We labeled the 3'UTR of the CaMKIIalpha mRNA in hippocampal cultures by using a green fluorescent protein (GFP)/MS2 bacteriophage tagging system. The CaMKIIalpha 3'UTR formed discrete granules throughout the dendrites of transfected cells. The identity of the fluorescent granules was verified by in situ hybridization. Over 30 min time periods these granules redistributed without a net increase in granule number; with depolarization there is a tendency toward increased numbers of granules in the dendrites. These observations suggest that finer time resolution of granule motility might reveal changes in the motility characteristics of granules after depolarization. So that motile granules could be tracked, shorter periods of observation were required. The movements of motile granules can be categorized as oscillatory, unidirectional anterograde, or unidirectional retrograde. Colocalization of CaMKIIalpha 3'UTR granules and synapses suggested that oscillatory movements allowed the granules to sample several local synapses. Neuronal depolarization increased the number of granules in the anterograde motile pool. Based on the time frame over which the granule number increased, the translocation of granules may serve to prepare the dendrite for mounting an adequate local translation response to future stimuli. Although the resident pool of granules can respond to signals that induce local translation, the number of granules in a dendrite might reflect its activation history.
pubmed:grant
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6385-93
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10964944-3' Untranslated Regions, pubmed-meshheading:10964944-Animals, pubmed-meshheading:10964944-Calcium-Calmodulin-Dependent Protein Kinase Type 2, pubmed-meshheading:10964944-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:10964944-Cells, Cultured, pubmed-meshheading:10964944-Cytoplasmic Granules, pubmed-meshheading:10964944-Dendrites, pubmed-meshheading:10964944-Embryo, Mammalian, pubmed-meshheading:10964944-Green Fluorescent Proteins, pubmed-meshheading:10964944-Hippocampus, pubmed-meshheading:10964944-Luminescent Proteins, pubmed-meshheading:10964944-Models, Neurological, pubmed-meshheading:10964944-Neurons, pubmed-meshheading:10964944-RNA, Messenger, pubmed-meshheading:10964944-Rats, pubmed-meshheading:10964944-Rats, Sprague-Dawley, pubmed-meshheading:10964944-Recombinant Fusion Proteins
pubmed:year
2000
pubmed:articleTitle
CaMKIIalpha 3' untranslated region-directed mRNA translocation in living neurons: visualization by GFP linkage.
pubmed:affiliation
Center for Neurological Diseases, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't