Linked Life Data

10964511

Source:http://linkedlifedata.com/resource/pubmed/id/10964511

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-9-22
pubmed:abstractText
Among the high-throughput, comprehensive technological methods used to analyze transcript expression levels, array-based hybridization and serial analysis of gene expression (SAGE) are currently the most common approaches. To compare the quantitative accuracy of oligonucleotide array and SAGE, both methods were carried out on identical RNA specimens prepared from human blood monocytes and granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced macrophages. For SAGE analysis, 57,560 and 57,463 tags were obtained from monocytes and macrophages, respectively, resulting in approximately 28,000 different tags, while oligo array hybridization was performed with GeneChip (Affymetrix), which represents approximately 6000 transcripts. These two methods correlated quite well in both absolute expression analyses and comparative analyses during differentiation. The correlation was better for genes with higher expression levels and greater changes in expression. This finding suggests that GeneChip technology is reasonably reliable for quantitative analysis of expression profiling and would be appropriate as a common platform upon which to build a gene expression database.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0888-7543
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
136-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Direct comparison of GeneChip and SAGE on the quantitative accuracy in transcript profiling analysis.
pubmed:affiliation
Genome Science Division, Research Center for Advanced Science and Technology, Tokyo, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't