Linked Life Data

10963811

Source:http://linkedlifedata.com/resource/pubmed/id/10963811

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-9-15
pubmed:abstractText
We studied the Th2/Th1 balance by short-term stimulation of peripheral blood mononuclear cells (PBMC) isolated during the pollen season from seven allergic patients treated with conventional birch-pollen immunotherapy (IT) for 18 months, eight matched allergic control patients and 10 non-atopic individuals. The PBMC were cultured for 7 days with birch-pollen extract (BPE) or tetanus toxoid (TT), and then restimulated with PHA and PMA to induce high IL-5, IL-10 and IFN-gamma production. The serum levels of birch-pollen-specific IgG and IgG4 were significantly elevated after IT treatment. The proliferative response to BPE was significantly enhanced in the allergic control group, but not in the IT-treated group, compared to the non-atopic group (P<0.05). Birch-pollen-specific IL-5 production was significantly enhanced in both the IT-treated group and the allergic control group (P<0.01-0. 05). Furthermore, both the IT-treated group and the allergic control group had a cytokine profile to BPE significantly more Th2 polarized (high IL-5/IFN-gamma ratio) than to TT (P<0.05 and P<0.01, respectively). No differences in IL-10 production between the three study groups were observed. The Th2/Th1 balance in vitro correlated with the serum concentrations of birch-pollen-specific IgE (r=0.60, P<0.05), and in the IT-treated group, also with the IgG and IgG4 levels (r=0.79, P<0.05 and r=0.86, P<0.05, respectively). We conclude that conventional birch-pollen IT does not lead to changes in the cytokine profile of the circulating pool of allergen-specific T cells during birch-pollen season. However, induction of peripheral T-cell tolerance and increased production of specific IgG and IgG4 might be part of the mechanisms of IT.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0165-2478
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
51-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10963811-Adult, pubmed-meshheading:10963811-Allergens, pubmed-meshheading:10963811-Cytokines, pubmed-meshheading:10963811-Desensitization, Immunologic, pubmed-meshheading:10963811-Female, pubmed-meshheading:10963811-Humans, pubmed-meshheading:10963811-Immune Tolerance, pubmed-meshheading:10963811-Immunoglobulin E, pubmed-meshheading:10963811-Immunoglobulin G, pubmed-meshheading:10963811-Interferon-gamma, pubmed-meshheading:10963811-Interleukin-10, pubmed-meshheading:10963811-Interleukin-5, pubmed-meshheading:10963811-Leukocytes, Mononuclear, pubmed-meshheading:10963811-Lymphocyte Activation, pubmed-meshheading:10963811-Male, pubmed-meshheading:10963811-Middle Aged, pubmed-meshheading:10963811-Phytotherapy, pubmed-meshheading:10963811-Pollen, pubmed-meshheading:10963811-Rhinitis, Allergic, Seasonal, pubmed-meshheading:10963811-Tetanus Toxoid, pubmed-meshheading:10963811-Th1 Cells, pubmed-meshheading:10963811-Th2 Cells, pubmed-meshheading:10963811-Trees
pubmed:year
2000
pubmed:articleTitle
Cytokine production by peripheral blood mononuclear cells following birch-pollen immunotherapy.
pubmed:affiliation
Department of Medical Sciences, Respiratory Medicine and Allergology, Uppsala University, Uppsala, Sweden.robert.moveare@eu.pnu.com
pubmed:publicationType
Journal Article, Clinical Trial, Controlled Clinical Trial