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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-10-16
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pubmed:abstractText |
Structural changes in subfragment 1 of skeletal muscle myosin were investigated by cross-linking trypsin-cleaved S1 with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. In the absence of nucleotide the alkali light chains are cross-linked to the 27 kDa heavy chain fragment; the presence of MgATP reduces the efficiency of this reaction. On the other hand, MgATP promotes the cross-link formation between the N-terminal 27 kDa and C-terminal 20 kDa fragments of the heavy chain. The chemical cleavage of the cross-linked heavy chains fragments with N-chlorosuccinimide and hydroxylamine indicates that the cross-links are formed between the regions spanning residues 131-204 and 699-809. These results indicate that the two regions of the heavy chain that are relatively distant in nucleotide-free skeletal S1 [Rayment et al. (1993) Science 261, 50-58] can potentially interact upon addition of nucleotide.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/9-anthroylnitrile,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Anthracenes,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Ethyldimethylaminopropyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Myosin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Myosin Light Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Myosin Subfragments,
http://linkedlifedata.com/resource/pubmed/chemical/Myosins,
http://linkedlifedata.com/resource/pubmed/chemical/N-chlorosuccinimide,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Succinimides,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
1481
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-62
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10962092-Adenosine Triphosphate,
pubmed-meshheading:10962092-Anthracenes,
pubmed-meshheading:10962092-Cross-Linking Reagents,
pubmed-meshheading:10962092-Ethyldimethylaminopropyl Carbodiimide,
pubmed-meshheading:10962092-Fluorescent Dyes,
pubmed-meshheading:10962092-Models, Molecular,
pubmed-meshheading:10962092-Myosin Heavy Chains,
pubmed-meshheading:10962092-Myosin Light Chains,
pubmed-meshheading:10962092-Myosin Subfragments,
pubmed-meshheading:10962092-Myosins,
pubmed-meshheading:10962092-Nucleotides,
pubmed-meshheading:10962092-Peptide Fragments,
pubmed-meshheading:10962092-Protein Conformation,
pubmed-meshheading:10962092-Succinimides,
pubmed-meshheading:10962092-Trypsin
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pubmed:year |
2000
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pubmed:articleTitle |
Nucleotide-induced movements in the myosin head near the converter region.
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pubmed:affiliation |
Department of Muscle Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur St., PL-02-093 Warsaw, Poland. basiap@nencki.gov.pl
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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