Source:http://linkedlifedata.com/resource/pubmed/id/10961387
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2000-9-7
|
| pubmed:abstractText |
Genistein, a prominent isoflavone in soy products, produced dose- and time-dependent in vitro growth inhibition at high concentrations (at least 185 microM) with an IC50 of 7.0-274.2 microM after 72 h incubation in four breast cancer cell lines (DD-762, Sm-MT, MCF-7 and MDA-MB-231) and one breast epithelial cell line (HBL- 100) of human and animal origin; it stimulated estrogen-receptor-positive MCF-7 cells at low concentrations (3.7 nM-37 microM). Genistein-exposed cells underwent apoptosis, confirmed by G2/M arrest followed by the appearance of a sub-G1 fraction in cell-cycle progression, and by a characteristic cell ultrastructure. The apoptosis cascade was due to up-regulation of Bax protein, down-regulation of Bcl-XL protein, and activation of caspase-3. Genistein acted in synergism with eicosapentaenoic acid (EPA), a fish oil component, on human breast cancer MCF-7 cells (genistein > 93.2 microM and EPA > 210.9 microM) and on MDA-MB-231 cells (genistein > 176.1 microM and EPA > 609.3 microM). Dietary intake of genistein in combination with EPA may be beneficial for breast cancer control.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0171-5216
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
126
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
448-54
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:10961387-Animals,
pubmed-meshheading:10961387-Apoptosis,
pubmed-meshheading:10961387-Breast Neoplasms,
pubmed-meshheading:10961387-Cell Cycle,
pubmed-meshheading:10961387-Diet,
pubmed-meshheading:10961387-Dose-Response Relationship, Drug,
pubmed-meshheading:10961387-Drug Synergism,
pubmed-meshheading:10961387-Eicosapentaenoic Acid,
pubmed-meshheading:10961387-Female,
pubmed-meshheading:10961387-Flow Cytometry,
pubmed-meshheading:10961387-Genistein,
pubmed-meshheading:10961387-Growth Inhibitors,
pubmed-meshheading:10961387-Humans,
pubmed-meshheading:10961387-Insectivora,
pubmed-meshheading:10961387-Mice,
pubmed-meshheading:10961387-Microscopy, Electron,
pubmed-meshheading:10961387-Receptors, Estrogen,
pubmed-meshheading:10961387-Tumor Cells, Cultured
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Effects of genistein and synergistic action in combination with eicosapentaenoic acid on the growth of breast cancer cell lines.
|
| pubmed:affiliation |
Department of Surgery II, Kansai Medical University, Moriguchi, Osaka, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|