Source:http://linkedlifedata.com/resource/pubmed/id/10960690
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2000-10-18
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pubmed:abstractText |
Previous studies have suggested a direct relationship between the nasopharyngeal carriage of potential middle ear pathogens and the development of middle ear disease. It has been shown that otitis-prone (OP) children tend to be colonized more often than non-OP children. To study the turnover of nontypeable Haemophilus influenzae (NTHI) in the nasopharynx of OP children, arbitrarily primed PCR was applied to NTHI strains isolated nasopharyngeal swabs collected prospectively during a 2-year study period from 35 OP children under 4 years of age at fixed intervals. METHODOLOGY/MATERIALS: In 20 patients, H. influenzae could be isolated from different sites (left and/or right ear and/or nasopharynx) or at different occasions during follow-up. Forty-eight H. influenzae isolates of different sites (left and/or right ear and/or nasopharynx) of the same patient as well as from siblings were typed using arbitrarily primed PCR with primer ERIC2 and RAPD Ready-to-Go beads (Pharmacia Biotech, Uppsala, Sweden).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0165-5876
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7-12
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:10960690-Child, Preschool,
pubmed-meshheading:10960690-Female,
pubmed-meshheading:10960690-Haemophilus Infections,
pubmed-meshheading:10960690-Haemophilus influenzae,
pubmed-meshheading:10960690-Humans,
pubmed-meshheading:10960690-Infant,
pubmed-meshheading:10960690-Male,
pubmed-meshheading:10960690-Nasopharynx,
pubmed-meshheading:10960690-Otitis Media,
pubmed-meshheading:10960690-Polymerase Chain Reaction,
pubmed-meshheading:10960690-Time Factors
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pubmed:year |
2000
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pubmed:articleTitle |
Turnover of Haemophilus influenzae isolates in otitis-prone children.
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pubmed:affiliation |
Department of Otolaryngology, University Hospital Ghent, De Pintelaan 185, B-9000, Ghent, Belgium. ingeborg.dhooge@rug.ac.be
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pubmed:publicationType |
Journal Article
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