Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1979-9-1
|
| pubmed:abstractText |
Requirements for helper T-cell recognition of H-2 determinants expressed on adherent accessory cells and on B cells was individually assessed in the anti-hapten PFC responses to TNP-KLH. Complicating allogeneic effects were minimized or avoided by the use of helper T cells from normal F1 hybrids, parent leads to F1 chimeras, and F1 leads to parent chimeras. The results of both in vitro and in vivo experiments demonstrated that: (a) helper T cells are not required to recognize the identical H-2 determinants on both accessory cells and B cells; (b) helper T cells are required to recognize K or I-A region-encoded determinants expressed on accessory cells; (c) no requirement was observed in vitro or in vivo for helper T-cell recognition of B-cell-expressed H-2 determinants; and (d) no requirement was observed for H-2 homology between accessory cells and B cells. The absence of required helper T-cell recognition of the identical H-2 determinants on both accessory cells and B cells was demonstrated in two ways: (a) naive of KLH-primed (A x B)F1 hybrid helper T cells collaborated equally well with B cells from either parentA or parentB in the presence of accessory cells from either parent; (b) A leads to (A x B)F1 chimeric spleen cells depleted of accessory cells collaborated equally well with accessory cells from either parentA or parentB, even though the B cells only expressed the H-2 determinants of parentA. A requirement for helper T-cell recognition of K or I-A region-encoded H-2 determinants on accessory cells was also demonstrated in two ways: (a) (A x B)F1 leads to parentA chimeric spleen cells depleted of accessory cells collaborated with accessory cells from parentA but not parentB; and (b) (A x B)F1 leads to parentA chimeric helper T cells collaborated with normal F1 B cells only in the presence of parental or recombinant accessory cells that expressed the K or I-A region-encoded determinants of parentA. Although restricted in their ability to recognize H-2 determinants on accessory cells, it was demonstrated both in vitro and in vivo that (A x B)F1 leads to parentA chimeric helper T cells were able to collaborate with B cells from either parentA or parentB. In vitro in the presence of accessory cells from parentA, (A x B)F1 leads to parentA chimeric helper T cells collaborated equally well with B cells from either parent. In addition, the inability of (A x B)F1 leads to parentA chimeric helper T cells to collaborate with (B + accessory) cells from parentB was successfully reversed by the addition of parentA SAC as added accessory cells. In vivo, upon the addition of parentA accessory cells, (A x B)F1 leads to parentA chimeric helper T cells collaborated with parentB B cells in short-term adoptive transfer experiments.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-102721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-1079849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-123544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-14029139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-154105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-304458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-305459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-305460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-306407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-306408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-308092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-4122709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-415885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-4542806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-4942407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-51901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-5355110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-621385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-62818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-68474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-75242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-77879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-81238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-956724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/109564-98611
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1007
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
149
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1208-26
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:109564-Animals,
pubmed-meshheading:109564-Antigens,
pubmed-meshheading:109564-B-Lymphocytes,
pubmed-meshheading:109564-Colony-Forming Units Assay,
pubmed-meshheading:109564-Crosses, Genetic,
pubmed-meshheading:109564-Genes, MHC Class II,
pubmed-meshheading:109564-Hemocyanin,
pubmed-meshheading:109564-Lymphocytes,
pubmed-meshheading:109564-Major Histocompatibility Complex,
pubmed-meshheading:109564-Male,
pubmed-meshheading:109564-Mice,
pubmed-meshheading:109564-T-Lymphocytes
|
| pubmed:year |
1979
|
| pubmed:articleTitle |
Cellular and genetic control of antibody responses. V. Helper T-cell recognition of H-2 determinants on accessory cells but not B cells.
|
| pubmed:publicationType |
Journal Article
|