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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-9-5
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pubmed:abstractText |
The AP-3 adaptor protein complex has been implicated in the biogenesis of lysosome-related organelles, such as pigment granules/melanosomes, and synaptic vesicles. Here we compare the relative importance of AP-3 in the biogenesis of these organelles in Drosophila melanogaster. We report that the Drosophila pigmentation mutants orange and ruby carry genetic lesions in the sigma3 and beta3-adaptin subunits of the AP-3 complex, respectively. Electron microscopy reveals dramatic reductions in the numbers of electron-dense pigment granules in the eyes of these AP-3 mutants. Mutant flies also display greatly reduced levels of pigments housed in these granules. In contrast, electron microscopy of retinula cells reveals numerous synaptic vesicles in both AP-3 mutant and wild-type flies, while behavioral assays show apparently normal locomotor ability of AP-3 mutant larvae. Together, these results demonstrate that Drosophila AP-3 is critical for the biogenesis of pigment granules, but is apparently not essential for formation of a major population of synaptic vesicles in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0026-8925
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1003-14
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10954086-Adaptor Protein Complex alpha Subunits,
pubmed-meshheading:10954086-Adaptor Proteins, Vesicular Transport,
pubmed-meshheading:10954086-Amino Acid Sequence,
pubmed-meshheading:10954086-Animals,
pubmed-meshheading:10954086-Behavior, Animal,
pubmed-meshheading:10954086-Cell Differentiation,
pubmed-meshheading:10954086-Cytoplasmic Granules,
pubmed-meshheading:10954086-Drosophila melanogaster,
pubmed-meshheading:10954086-Genes, Insect,
pubmed-meshheading:10954086-Membrane Proteins,
pubmed-meshheading:10954086-Molecular Sequence Data,
pubmed-meshheading:10954086-Monomeric Clathrin Assembly Proteins,
pubmed-meshheading:10954086-Movement,
pubmed-meshheading:10954086-Mutation,
pubmed-meshheading:10954086-Photoreceptor Cells, Invertebrate,
pubmed-meshheading:10954086-Pigments, Biological,
pubmed-meshheading:10954086-Sequence Homology, Amino Acid,
pubmed-meshheading:10954086-Synaptic Vesicles
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pubmed:year |
2000
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pubmed:articleTitle |
Distinct requirements for the AP-3 adaptor complex in pigment granule and synaptic vesicle biogenesis in Drosophila melanogaster.
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pubmed:affiliation |
Cell Biology and Metabolism Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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