Source:http://linkedlifedata.com/resource/pubmed/id/10950986
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 9
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pubmed:dateCreated |
2000-11-9
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pubmed:abstractText |
Porcine circovirus 2 (PCV2), a single-stranded DNA virus associated with post-weaning multisystemic wasting syndrome of swine, has two potential open reading frames, ORF1 and ORF2, greater than 600 nucleotides in length. ORF1 is predicted to encode a replication-associated protein (Rep) essential for replication of viral DNA, while ORF2 contains a conserved basic amino acid sequence at the N terminus resembling that of the major structural protein of chicken anaemia virus. Thus far, the structural protein(s) of PCV2 have not been identified. In this study, a viral structural protein of 30 kDa was identified in purified PCV2 particles. ORF2 of PCV2 was cloned into a baculovirus expression vector and the gene product was expressed in insect cells. The expressed ORF2 gene product had a molecular mass of 30 kDa, similar to that detected in purified virus particles. The recombinant ORF2 protein self-assembled to form capsid-like particles when viewed by electron microscopy. Antibodies against the ORF2 protein were detected in samples of sera obtained from pigs as early as 3 weeks after experimental infection with PCV2. These results show that the major structural protein of PCV2 is encoded by ORF2 and has a molecular mass of 30 kDa.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Structural Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/replication initiator protein
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1317
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2281-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10950986-Animals,
pubmed-meshheading:10950986-Baculoviridae,
pubmed-meshheading:10950986-Capsid,
pubmed-meshheading:10950986-Cells, Cultured,
pubmed-meshheading:10950986-Circovirus,
pubmed-meshheading:10950986-Cloning, Molecular,
pubmed-meshheading:10950986-DNA Helicases,
pubmed-meshheading:10950986-DNA Replication,
pubmed-meshheading:10950986-DNA-Binding Proteins,
pubmed-meshheading:10950986-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10950986-Molecular Weight,
pubmed-meshheading:10950986-Open Reading Frames,
pubmed-meshheading:10950986-Spodoptera,
pubmed-meshheading:10950986-Swine,
pubmed-meshheading:10950986-Trans-Activators,
pubmed-meshheading:10950986-Viral Proteins,
pubmed-meshheading:10950986-Viral Structural Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein.
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pubmed:affiliation |
Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 1802 Elwood Drive, Bldg 6, Ames, 50011-1240, USA.
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pubmed:publicationType |
Journal Article
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