Linked Life Data

10950189

Source:http://linkedlifedata.com/resource/pubmed/id/10950189

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-11-22
pubmed:abstractText
A rapid screening technique for isolation of selenite (Se(IV)) reduction-deficient (Ser) mutants was developed and used to identify four Ser mutants of Shewanella putrefaciens. Two Ser mutants were unable to grow anaerobically on fumarate, nitrate or nitrite. Two other Ser mutants were unable to grow anaerobically on all compounds tested as sole terminal electron acceptor. Previously isolated Mn(IV) reduction-deficient mutants displayed Ser-positive phenotypes and reduced Se(IV) at wild-type rates, while two of nine Fe(III) reduction-deficient mutants displayed Ser-negative phenotypes and reduced Se(IV) at low rates. This study provides the first reported method for isolation of Ser mutants and demonstrates that Se(IV) reduction by S. putrefaciens is respiratory chain-linked.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0944-5013
pubmed:author
pubmed:issnType
Print
pubmed:volume
155
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
79-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Design and application of a rapid screening technique for isolation of selenite reduction-deficient mutants of Shewanella putrefaciens.
pubmed:affiliation
School of Biology, Georgia Institute of Technology, Atlanta 30332, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't