Linked Life Data

10946286

Source:http://linkedlifedata.com/resource/pubmed/id/10946286

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-9-19
pubmed:databankReference
pubmed:abstractText
Chemokines play important roles in leukocyte trafficking as well as function regulation. In this study, we described the identification and characterization of a novel CXC chemokine from a human dendritic cell (DC) cDNA library, the full-length cDNA of which contains an open reading frame encoding 111 aa with a putative signal peptide of 34 aa. This CXC chemokine shares greatest homology with macrophage inflammatory protein (MIP)-2alphabeta, hence is designated as MIP-2gamma. Mouse MIP-2gamma was identified by electrocloning and is highly homologous to human MIP-2gamma. Northern blotting revealed that MIP-2gamma was constitutively and widely expressed in most normal tissues with the greatest expression in kidney, but undetectable in most tumor cell lines except THP-1 cells. In situ hybridization analysis demonstrated that MIP-2gamma was mainly expressed by the epithelium of tubules in the kidney and hepatocytes in the liver. Although no detectable expression was observed in freshly isolated or PMA-treated monocytes, RT-PCR analysis revealed MIP-2gamma expression by monocyte-derived DC. Recombinant MIP-2gamma from 293 cells is about 9.5 kDa in size and specifically detectable by its polyclonal Ab developed by the immunization with its 6His-tagged fusion protein. The eukaryotically expressed MIP-2gamma is a potent chemoattractant for neutrophils, and weaker for DC, but inactive to monocytes, NK cells, and T and B lymphocytes. Receptor binding assays showed that MIP-2gamma does not bind to CXCR2. This implies that DC might contribute to the innate immunity through the production of neutrophil-attracting chemokines and extends the knowledge about the regulation of DC migration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2588-95
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10946286-Adult, pubmed-meshheading:10946286-Amino Acid Sequence, pubmed-meshheading:10946286-Animals, pubmed-meshheading:10946286-Base Sequence, pubmed-meshheading:10946286-Cells, Cultured, pubmed-meshheading:10946286-Chemokine CXCL2, pubmed-meshheading:10946286-Chemokines, CXC, pubmed-meshheading:10946286-Chemotaxis, Leukocyte, pubmed-meshheading:10946286-Cloning, Molecular, pubmed-meshheading:10946286-DNA, Complementary, pubmed-meshheading:10946286-Dendritic Cells, pubmed-meshheading:10946286-Genetic Vectors, pubmed-meshheading:10946286-Humans, pubmed-meshheading:10946286-Mice, pubmed-meshheading:10946286-Molecular Sequence Data, pubmed-meshheading:10946286-Monokines, pubmed-meshheading:10946286-Neutrophils, pubmed-meshheading:10946286-Organ Specificity, pubmed-meshheading:10946286-Protein Binding, pubmed-meshheading:10946286-Receptors, Chemokine, pubmed-meshheading:10946286-Receptors, Interleukin, pubmed-meshheading:10946286-Receptors, Interleukin-8B, pubmed-meshheading:10946286-Recombinant Proteins, pubmed-meshheading:10946286-Transfection, pubmed-meshheading:10946286-Tumor Cells, Cultured
pubmed:year
2000
pubmed:articleTitle
Molecular cloning and characterization of a novel CXC chemokine macrophage inflammatory protein-2 gamma chemoattractant for human neutrophils and dendritic cells.
pubmed:affiliation
Department of Immunology and Shanghai Brilliance Biotechnology Institute, Second Military Medical University, Shanghai, People's Republic of China. caoxt@public3.sta.net.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't