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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-11-8
pubmed:databankReference
pubmed:abstractText
Protein phosphatase 2A (PP2A) is one kind of serine/ threonine protein phosphatase regulating mainly cell growth and division. It comprises three subunits, A, B, and C. The B-subunit is involved in enzyme activity and substrate specificity. The B-subunit family is of great diversity and is divided into three classes, the B1, B2, and B3 subfamilies. Until now, two members of the B1 subfamily, B1alpha and B1beta, have been identified in human. In this report, the third member of the sub-family, B1gamma, was identified, and its cDNA was isolated from a human brain cDNA library. This novel cDNA is 4,120 bp in length and contains an open reading frame (nt 55-1,398) encoding 447 amino acid residues. The putative protein shares 81 and 85% identity with B1alpha (PPP2R2A) and B1beta (PPP2R2B), respectively, and was named PPP2R2C for its high level of homology to the other two isoforms. One remarkable characteristic of this novel gene is that it is highly expressed in brain with a 4.7-kb transcript while it is nearly undetectable in other tissues. In addition, the PPP2R2C gene was localized to human chromosome 4p16 between markers D4S2925 and D4S3007 with 5.45 cR (LOD > 14) and 2.63 cR (LOD > 15) RH distance, respectively, by radiation hybrid panel mapping.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0888-7543
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10945473-Amino Acid Sequence, pubmed-meshheading:10945473-Base Sequence, pubmed-meshheading:10945473-Blotting, Northern, pubmed-meshheading:10945473-Brain, pubmed-meshheading:10945473-Chromosomes, Human, Pair 4, pubmed-meshheading:10945473-Cloning, Molecular, pubmed-meshheading:10945473-DNA, Complementary, pubmed-meshheading:10945473-DNA Primers, pubmed-meshheading:10945473-Humans, pubmed-meshheading:10945473-Isoenzymes, pubmed-meshheading:10945473-Lod Score, pubmed-meshheading:10945473-Molecular Sequence Data, pubmed-meshheading:10945473-Phosphoprotein Phosphatases, pubmed-meshheading:10945473-Polymerase Chain Reaction, pubmed-meshheading:10945473-Protein Phosphatase 2, pubmed-meshheading:10945473-Radiation Hybrid Mapping, pubmed-meshheading:10945473-Sequence Homology, Amino Acid, pubmed-meshheading:10945473-Tissue Distribution
pubmed:year
2000
pubmed:articleTitle
Molecular cloning and mapping of the brain-abundant B1gamma subunit of protein phosphatase 2A, PPP2R2C, to human chromosome 4p16.
pubmed:affiliation
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't