Linked Life Data

10945341

Source:http://linkedlifedata.com/resource/pubmed/id/10945341

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-11-21
pubmed:abstractText
The reasons for the presence of a multitude of plasma membrane-localized water channels (PIP aquaporins) in plants may be functional differences in water (or other solute) transport, or in developmental, environmental or tissue-specific regulation of expression. We compared tissue- and cell-specific expression of McMipA, an abundantly expressed PIP from the common ice plant (Mesembryanthemum crystallinum), with that of the previously characterized McMipB [Yamada et al. (1997) Plant Cell Physiol. 38: 1326]. The activity of a 2.2 kb DNA fragment containing the promoter region of McMipA in a fusion with the GUS coding region was studied in transgenic tobacco. The McMipA promoter was active in pericycle and cortex cells in roots and in phloem-associated cells and cells surrounding the pericycle in shoots. In green leaves, mesophyll cells and the minor veins showed GUS activity, but the major veins did not. In floral tissues, GUS activity was observed in the pistil and anthers of immature buds and the tip of the mature pistil and pollen. Neither the apical meristem nor root tip showed any GUS activity. The differences in tissue specificity between the McMipA and McMipB promoters indicated that the two PIPs, MC-MIPA and MC-MIPB, serve different functions in plants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0032-0781
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
719-25
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Expression of the PIP aquaporin promoter-MipA from the common ice plant in tobacco.
pubmed:affiliation
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc, Iwata, Shizuoka.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.