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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-9-21
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pubmed:abstractText |
Dityrosine is one of the specific biomarkers for proteinoxidation. We prepared an antibody specific for proteindityrosine using a dimer of 3-p-(hydroxyphenyl)propionic acid (di-HP) as a hapten. Three clones (A8, G6, and 1C3) were obtained, and the antibody from the A8 clone reacted with the di-HP-conjugated protein but not with a dityrosine conjugate. The others (G6 and 1C3 clones) recognized both the di-HP and dityrosine conjugates. The antibodies reacted with peptidyl dityrosine, derived from Thr-Tyr-Ser, rather than the free dityrosine. The reactivity of the latter two antibodies with lens proteins oxidized by incubation with H(2)O(2)/Cu was in good accordance with the formation of the dityrosine-like fluorescence. Using the obtained monoclonal antibody, the immunopositive staining of atherosclerotic lesions in Apo E-deficient mice was confirmed by an immunohistochemical technique.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins E,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Crystallins,
http://linkedlifedata.com/resource/pubmed/chemical/Haptens,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylpropionates,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/dityrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11-5
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:10944432-Animals,
pubmed-meshheading:10944432-Antibodies, Monoclonal,
pubmed-meshheading:10944432-Antibody Specificity,
pubmed-meshheading:10944432-Antigens,
pubmed-meshheading:10944432-Aorta, Thoracic,
pubmed-meshheading:10944432-Apolipoproteins E,
pubmed-meshheading:10944432-Arteriosclerosis,
pubmed-meshheading:10944432-Binding, Competitive,
pubmed-meshheading:10944432-Biological Markers,
pubmed-meshheading:10944432-Crystallins,
pubmed-meshheading:10944432-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10944432-Haptens,
pubmed-meshheading:10944432-Hydrogen Peroxide,
pubmed-meshheading:10944432-Immune Sera,
pubmed-meshheading:10944432-Immunohistochemistry,
pubmed-meshheading:10944432-Male,
pubmed-meshheading:10944432-Mice,
pubmed-meshheading:10944432-Mice, Inbred BALB C,
pubmed-meshheading:10944432-Molecular Weight,
pubmed-meshheading:10944432-Oxidants,
pubmed-meshheading:10944432-Phenylpropionates,
pubmed-meshheading:10944432-Tyrosine
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pubmed:year |
2000
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pubmed:articleTitle |
Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody.
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pubmed:affiliation |
School of Humanities for Environmental Policy and Technology, Himeji Institute of Technology, Himeji, 670-0092, Japan.
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pubmed:publicationType |
Journal Article
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