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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-11-29
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pubmed:abstractText |
The effects of Matrigel, a reconstituted basement membrane, on human granulosa cells were investigated. Cells were obtained from follicular aspirate in the course of oocyte retrieval for in vitro fertilization and were cultured on either a surface coated with Matrigel or uncoated plastic. Light and electron microscopy showed that granulosa cells cultured on Matrigel demonstrated three-dimensional aggregated cells with well differentiated morphology: numerous lipid droplets, microvilli, junctional complexes and lumen-like structures were seen. In contrast, cells cultured on plastic were flattened, poorly differentiated and showed apoptotic cells. Immunocytochemistry showed that the proportion of immunopositive cells for 3beta-hydroxysteroid dehydrogenase was increased in cultures on Matrigel. The results of the present study suggest that culture on Matrigel promotes the differentiation of human granulosa cells and provides a useful tool which may improve the efficiency of in vitro fertilization.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0918-8959
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-83
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10943742-3-Hydroxysteroid Dehydrogenases,
pubmed-meshheading:10943742-Apoptosis,
pubmed-meshheading:10943742-Cell Adhesion,
pubmed-meshheading:10943742-Cell Differentiation,
pubmed-meshheading:10943742-Cells, Cultured,
pubmed-meshheading:10943742-Collagen,
pubmed-meshheading:10943742-Culture Media,
pubmed-meshheading:10943742-Drug Combinations,
pubmed-meshheading:10943742-Female,
pubmed-meshheading:10943742-Granulosa Cells,
pubmed-meshheading:10943742-Humans,
pubmed-meshheading:10943742-Immunohistochemistry,
pubmed-meshheading:10943742-Intercellular Junctions,
pubmed-meshheading:10943742-Laminin,
pubmed-meshheading:10943742-Lipids,
pubmed-meshheading:10943742-Microscopy, Electron,
pubmed-meshheading:10943742-Microvilli,
pubmed-meshheading:10943742-Proteoglycans
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pubmed:year |
2000
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pubmed:articleTitle |
Role of reconstituted basement membrane in human granulosa cell culture.
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pubmed:affiliation |
Department of Anatomy, College of Medicine, Seoul National University, Jongro-Gu, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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