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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2000-12-6
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pubmed:abstractText |
Secretion of Monocyte Chemotactic Protein-1 (MCP-1) by fibroblasts infected with Toxoplasma gondii was studied in vitro. A significantly higher MCP-1 secretion was observed 24 h after infection by live tachyzoites. Analysis of chemokine mRNA transcripts by RNase protection assay revealed that this MCP-1 secretion seems associated with increased MCP-1 mRNA expression. However, these increased levels of MCP-1 secretion and expression were not obtained after stimulation by heat-killed tachyzoites or parasites pre-treated by a specific inhibitor of phosphatidylcholine-specific phospholipase C (D609). Inhibition of parasite multiplication by pyrimethamine did not modify MCP-1 secretion. Thus, it appeared that the active penetration of T. gondii in cells was of major importance in the induction of MCP-1 secretion. None of the other chemokines studied by RNase protection assay (lymphotactin, RANTES, IP-10, MIP-1alpha, MIP-1beta, IL-8, and I-309) were expressed after infection by live tachyzoites. We also found that MCP-1 secretion induced by live T. gondii is blocked by inhibitors of nuclear factor (NF)-kappaB activation, ALLN and MG132. Such data indicate that NF-kappaB could be involved in T. gondii-induced MCP-1 production. MCP-1 secretion may contribute to the recruitment of monocytes and lymphocytes and thus participate in the control of T. gondii infection and in its pathogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antiprotozoal Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Bridged Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Leupeptins,
http://linkedlifedata.com/resource/pubmed/chemical/Proline,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimethamine,
http://linkedlifedata.com/resource/pubmed/chemical/Thiocarbamates,
http://linkedlifedata.com/resource/pubmed/chemical/Thiones,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/benzyloxycarbonylleucyl-leucyl-leuci...,
http://linkedlifedata.com/resource/pubmed/chemical/prolinedithiocarbamate,
http://linkedlifedata.com/resource/pubmed/chemical/tricyclodecane-9-yl-xanthogenate
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0300-8177
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
209
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-87
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10942204-Animals,
pubmed-meshheading:10942204-Antiprotozoal Agents,
pubmed-meshheading:10942204-Bridged Compounds,
pubmed-meshheading:10942204-Cells, Cultured,
pubmed-meshheading:10942204-Chemokine CCL2,
pubmed-meshheading:10942204-Chemokines,
pubmed-meshheading:10942204-Enzyme Inhibitors,
pubmed-meshheading:10942204-Fibroblasts,
pubmed-meshheading:10942204-Gene Expression Regulation,
pubmed-meshheading:10942204-Humans,
pubmed-meshheading:10942204-Kinetics,
pubmed-meshheading:10942204-Leupeptins,
pubmed-meshheading:10942204-Mice,
pubmed-meshheading:10942204-Proline,
pubmed-meshheading:10942204-Pyrimethamine,
pubmed-meshheading:10942204-Thiocarbamates,
pubmed-meshheading:10942204-Thiones,
pubmed-meshheading:10942204-Time Factors,
pubmed-meshheading:10942204-Toxoplasma,
pubmed-meshheading:10942204-Transcription, Genetic,
pubmed-meshheading:10942204-Type C Phospholipases
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pubmed:year |
2000
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pubmed:articleTitle |
Toxoplasma gondii induces the secretion of monocyte chemotactic protein-1 in human fibroblasts, in vitro.
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pubmed:affiliation |
Département de Parasitologie-Mycologie Médicale et Moléculaire (RHAP- DP3M) UPRES-A CNRS 5082, Faculté de Médecine, Université Joseph Fourier, Grenoble 1, La Tronche, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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