Linked Life Data

10941835

Source:http://linkedlifedata.com/resource/pubmed/id/10941835

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8-9
pubmed:dateCreated
2000-9-14
pubmed:databankReference
pubmed:abstractText
Human killer-cell immunoglobulin-like receptors (KIR) show three types of organization of their extracellular domains: D0-D1-D2 in KIR3D, D1-D2 in the majority of KIR2D, and D0-D2 in KIR2DL4 and the novel KIR2DL5. The gene for a KIR2DL3 variant, which has a D1-D2 structure, has been shown previously to have a nonexpressed region (pseudoexon 3) that is paralogous to the exon encoding the D0 domain of other KIR. This pseudoexon is not expressed because it is skipped during splicing of pre-mRNA. In this study, we demonstrate that all eight genes encoding human KIR with D1-D2 configuration (KIR2DL1-KIR2DL3, KIR2DS1-KIR2DS5) have similarly untranslated pseudoexons. Whereas the pseudoexons of four of these KIR genes bear nonsense mutations and/or altered splicing sites, the pseudoexons in the other four KIR genes have no major structural abnormalities, indicating that other mechanisms are responsible for inactivation of their exons 3. A comparison of the sequences on pseudoexons 3 with the paralogous expressed exons suggests that an exonic splicing enhancer may be necessary for the expression of exon 3 in KIR genes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0093-7711
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
639-46
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10941835-Adult, pubmed-meshheading:10941835-Base Sequence, pubmed-meshheading:10941835-DNA, Complementary, pubmed-meshheading:10941835-Exons, pubmed-meshheading:10941835-Humans, pubmed-meshheading:10941835-Killer Cells, Natural, pubmed-meshheading:10941835-Molecular Sequence Data, pubmed-meshheading:10941835-Protein Biosynthesis, pubmed-meshheading:10941835-Protein Structure, Tertiary, pubmed-meshheading:10941835-Pseudogenes, pubmed-meshheading:10941835-Receptors, Immunologic, pubmed-meshheading:10941835-Receptors, KIR, pubmed-meshheading:10941835-Receptors, KIR2DL1, pubmed-meshheading:10941835-Receptors, KIR2DL3, pubmed-meshheading:10941835-Receptors, KIR2DL4, pubmed-meshheading:10941835-Sequence Homology, Nucleic Acid, pubmed-meshheading:10941835-Tumor Cells, Cultured
pubmed:year
2000
pubmed:articleTitle
Genes encoding human killer-cell Ig-like receptors with D1 and D2 extracellular domains all contain untranslated pseudoexons encoding a third Ig-like domain.
pubmed:affiliation
Department of Structural Biology, Stanford University School of Medicine, CA 94305, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't