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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2000-9-13
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pubmed:abstractText |
Oligodeoxynucleotides (ODN) containing CpG motifs activate RAW 264.7 mouse macrophages and RPMI 8226 human myeloma cells to produce IL-12 p40. Using deletion and site-directed mutagenesis, the nuclear factor (NF)-kappaB half-site and the CCAAT/enhancer binding protein (C/EBP) recognition site were identified as potent cis-acting elements in CpG ODN-mediated IL-12 p40 promoter activation. Several NF-kappaB/Rel proteins competed for binding to the NF-kappaB half-site. The p65/c-Rel and p65/p50 heterodimer occupied this site shortly after CpG ODN administration (0.5-2 h), while the p50/c-Rel heterodimer dominated binding in the late stage (8-12 h). The induction of p50/c-Rel heterodimer was associated with a significant expression of IL-12 p40 mRNA. C/EBPbeta also contributed to CpG ODN-mediated IL-12 p40 promoter activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CPG-oligonucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B p50 Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-rel,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor RelA
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1967-76
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10940886-Adjuvants, Immunologic,
pubmed-meshheading:10940886-Animals,
pubmed-meshheading:10940886-Binding Sites,
pubmed-meshheading:10940886-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:10940886-Cell Line,
pubmed-meshheading:10940886-DNA-Binding Proteins,
pubmed-meshheading:10940886-Dimerization,
pubmed-meshheading:10940886-Gene Expression Regulation,
pubmed-meshheading:10940886-Humans,
pubmed-meshheading:10940886-Interleukin-12,
pubmed-meshheading:10940886-Macrophages,
pubmed-meshheading:10940886-Mice,
pubmed-meshheading:10940886-NF-kappa B,
pubmed-meshheading:10940886-NF-kappa B p50 Subunit,
pubmed-meshheading:10940886-Nuclear Proteins,
pubmed-meshheading:10940886-Oligodeoxyribonucleotides,
pubmed-meshheading:10940886-Oligonucleotides,
pubmed-meshheading:10940886-Promoter Regions, Genetic,
pubmed-meshheading:10940886-Proto-Oncogene Proteins c-rel,
pubmed-meshheading:10940886-Thionucleotides,
pubmed-meshheading:10940886-Transcription, Genetic,
pubmed-meshheading:10940886-Transcription Factor RelA,
pubmed-meshheading:10940886-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
CpG ODN-mediated regulation of IL-12 p40 transcription.
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pubmed:affiliation |
Section of Retroviral Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda 20892, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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