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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2000-9-13
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pubmed:abstractText |
Immunostimulatory DNA sequences (ISS) are a potent Th1 adjuvant. We hypothesized that conjugation of ISS to protein antigens would strongly enhance their immunogenicity because both antigen and adjuvant (ISS) would be delivered to the same locale/antigen-presenting cell. To test this hypothesis, we conjugated a 22-mer immunostimulatory oligodeoxynucleotide (ISS-ODN) to two test antigens of differing intrinsic immunogenicity, namely Escherichia coli beta-galactosidase and the HIV-1 envelope glycoprotein gp120. We show that the antigen-ISS conjugates rapidly induce Th1 cells secreting high levels of IFN-gamma, strong CTL activity, and high titer IgG2a and HIV-neutralizing antibodies, exceeding gene and protein vaccination alone or immunization with mixtures of antigen and ISS-ODN. The data suggest that this procedure generates a novel and unique vaccine that rapidly triggers strong humoral and cell-mediated immunity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-2980
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1939-47
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10940883-Animals,
pubmed-meshheading:10940883-Antibodies, Bacterial,
pubmed-meshheading:10940883-Antibody Formation,
pubmed-meshheading:10940883-CHO Cells,
pubmed-meshheading:10940883-Cricetinae,
pubmed-meshheading:10940883-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10940883-Escherichia coli,
pubmed-meshheading:10940883-Female,
pubmed-meshheading:10940883-HIV Antibodies,
pubmed-meshheading:10940883-HIV Envelope Protein gp120,
pubmed-meshheading:10940883-Immunity, Cellular,
pubmed-meshheading:10940883-Immunoglobulin G,
pubmed-meshheading:10940883-Interferon-gamma,
pubmed-meshheading:10940883-Mice,
pubmed-meshheading:10940883-Mice, Inbred BALB C,
pubmed-meshheading:10940883-Oligodeoxyribonucleotides,
pubmed-meshheading:10940883-Plasmids,
pubmed-meshheading:10940883-Sodium Dodecyl Sulfate,
pubmed-meshheading:10940883-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:10940883-Th1 Cells,
pubmed-meshheading:10940883-Thionucleotides,
pubmed-meshheading:10940883-Time Factors,
pubmed-meshheading:10940883-beta-Galactosidase
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pubmed:year |
2000
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pubmed:articleTitle |
Conjugation of protein to immunostimulatory DNA results in a rapid, long-lasting and potent induction of cell-mediated and humoral immunity.
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pubmed:affiliation |
Department of Medicine, The Sam and Rose Stein Institute for Research of Aging, University of California, San Diego, La Jolla 92093-0663, USA. htighe@ucsd.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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